Hi Vikki,

I don't know if I'm much help on this because I just started testing the BondMax this week so I'm just in the learning process too.  As far as I can tell the post primary is for blocking, but also works in conjunction with the polymer which is the following step.  It supposedly also enhances penetration of the polymer, so they both have to be used together.  Hope this helps.

Sharon


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker
Sent: Friday, August 01, 2008 1:13 PM
To: Histo Net list server
Subject: [Histonet] A question for BondMax users


Happy Friday everyone!

In the BondMax detection system there is a "post primary" step that contains 10% BSA in a Tris buffer.  I've tested the protocol excluding this step and did not have any staining.

I can't seem to get the answer I'm looking for from the company, so if anyone can help me I'd really appreciate it.

Thanks

Vikki

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