Consistent with my previous discussion on WGA-HRP use for anterograde tracing, I have purchased 1 mg L7017 from Sigma.
Given the tiny amount within the tube, do anyone have the experience in choosing a proper dilution factor for storage?
I think the best way is to dilute WGA-HRP into work solution, for example, 1% for axon tracing, and stored in separate tube (5 ul per tube)?
Or there are any other tricks?
They do not provide this on information sheet.
Thanks very much.
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