[Histonet] RE: Subscribe please

From:"Sue Ford"





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Sent: 29 July 2008 01:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 56, Issue 33


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Today's Topics:

   1. Immuno Stainer (Sandra Cheasty)
   2. Used embedding center (Va Paula Sicurello)
   3. RE: Alcohol lamps (Bonner, Janet)
   4. RE: Alcohol lamps (Thomas Jasper)
   5. Blue Zones (Okland, Gloria A)
   6. alcohol lamps (sharon.osborn@comcast.net)
   7. Re: Alcohol lamps (Gayle Callis)
   8. RE: Alcohol lamps (Monfils, Paul)
   9. Re: alcohol lamps (Jackie M O'Connor)
  10. Re: Scale for weighing parathyroid (Robert Richmond)
  11. RE: Alcohol lamps (Rittman, Barry R)
  12. RE: alcohol lamps (Smith, Allen)
  13. RE: alcohol lamps (Jackie M O'Connor)
  14. Human Herpes Virus 6 (Drew Sally A.)
  15. Re: alcohol lamps (Victor Tobias)
  16. Re: Human Herpes Virus 6 (tbritten@aol.com)
  17. Creutz Jacob Disease Fitxtion, Hanling, and disposal of
      infected tissue (Robert Fauck [CCDHB])
  18. Sodium barbital buffer substitute (Barbara Albert)
  19. RE: Alcohol lamps (Tony Henwood)


----------------------------------------------------------------------

Message: 1
Date: Mon, 28 Jul 2008 12:07:19 -0500
From: "Sandra Cheasty" 
Subject: [Histonet] Immuno Stainer
To: "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

Any suggestions re: an "open" Immunostainer that allows multiple detection kits, and also allows starting a new run before the previous run has completed? I work with  veterinary diagnostic and research tissues.

 

Thank you,

Sandy

UW-Madison

 

 

 

 





------------------------------

Message: 2
Date: Mon, 28 Jul 2008 10:44:45 -0700 (PDT)
From: Va Paula Sicurello 
Subject: [Histonet] Used embedding center
To: histonet@lists.utsouthwestern.edu
Message-ID: <214245.32808.qm@web46112.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Hi HistoPeople,

I'm looking for a used, but one that works, embedding center.  I'm in the process of adding a histology service to my EM and confocal lab and have everything except the embedding center.

If you have one laying around in a closet somewhere, I'll pay for shipping.

Please contact me if you have one that you'd like to get rid of.

Paula Sicurello
VA San Diego Healthcare System
Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


      




------------------------------

Message: 3
Date: Mon, 28 Jul 2008 13:43:47 -0400
From: "Bonner, Janet" 
Subject: RE: [Histonet] Alcohol lamps
To: "Peterson, Dan" <1dpeterson@meriter.com>,
	histonet@pathology.swmed.edu
Message-ID:
	<5F31F38C96781A4FBE3196EBC22D47807F2726@fhosxchmb006.ADVENTISTCORP.NET>
	
Content-Type: text/plain; charset=iso-8859-1

...and what about those xylene fumes that us old-timers cannot detect with our burned out noses?  A flame will ignite xylene as well.  Otherwise, we could all go back to "smokin' in da lab"!!
 
Janet L. Bonner, HTL (ASCP)
Pathology Laboratory
Florida Hospital Winter Park
janet.bonner@FLHOSP.org  
407-646-7559

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Peterson, Dan
Sent: Mon 7/28/2008 12:21 PM
To: histonet@pathology.swmed.edu
Subject: [Histonet] Alcohol lamps



Fellow Histonetters,
I am in disagreement with our lab's Safety officer with regards to
alcohol lamps. We use them in our embedding area to keep our forceps
clean.
The officer says that they're a fire hazard (even though we've used them
without incident for over 30 yrs). There are no flammable reagents
(other than the alcohol in the lamp) near the embedding area. I know we
could use the warming wells on the embedders, but try to find more that
1 pair of forceps that you like, or better yet, try to find a forceps
that the tech before hasn't left paraffin all over it. (Yes, I am a
fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY
touches)
Petty issue? Are there others out there using lamps? I am willing to
change if necessary (or so ordered), but would like to hear from those
who do the work, not be told what to do by those who know nothing of the
work. Thanks in advance!!

Daniel R Peterson HT(ASCP)
Histopathology Section Head
Meriter Laboratories
(608)-417-6557
1dpeterson@meriter.com

CONFIDENTIALITY NOTICE: This message (including any attachments) is
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hereby notified that any disclosure, copying or distribution of this
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------------------------------

Message: 4
Date: Mon, 28 Jul 2008 11:05:51 -0700
From: "Thomas Jasper" 
Subject: RE: [Histonet] Alcohol lamps
To: "Peterson, Dan" <1dpeterson@meriter.com>
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	<90354A475B420441B2A0396E5008D4965E211F@copc-sbs.COPC.local>
Content-Type: text/plain; charset="US-ASCII"

Hey Dan,

Am compelled to respond as I know just what you mean about having a
flame available.  Back in the "good old days" about 10 years ago, at my
previous employer, we were forced to get rid of our Bunsen burners.
These of course provided a flame just like your alcohol lamps.  I do
have to say they may have been a bit more dangerous as they were fed by
a tube of gas...I'm sure you get the picture.  Anyway, not only do I
agree with you about one forceps and the paraffin coating deal.  I also
liked the fact that the flame eliminated any residuals, between blocks.
I always thought that was a GLP re: cross contamination, floaters, etc.
However overall lab safety trumped my GLP reasons.  And as you've
stated...no accidents, ever to my knowledge, in my old lab.

I have to admit, when we lost the burners I had a sad feeling.  It may
seem a bit silly, but I felt it really changed how efficiently and
effectively we could get our embedding done.  Eventually we obtained
some bacti-incinerators (I think this has been mentioned already).
They're ok, but you've really got to watch it as the forceps heat so
quickly you'll burn your hand.  It'd be nice if you could get something
like a bacti-incinerator at a few thousand degrees cooler!?

So Dan, not that my story helps your cause any, but at least you know I
sympathize.  I do have the feeling your safety officer is probably going
to be able to throw his/her weight around with you on this one.  Where
I'm working now, we don't have bacti-incinerators.  We only have the
warming wells, and I try and rotate 2 or 3 of my "favorite" pairs of
forceps when embedding.  I know I'd be devastated if there'd ever been
an accident with flammables (as anyone would).  And I'm sure your safety
officer will make the point about paraffin being flammable near your
alcohol lamp.  I believe safety officers are forced to look at things a
certain way. There's no way he/she is ever going to understand where
you're coming from and probably has a hard time understanding what your
problem is.  But it's not their job to get your job done...what to do?
Think you'll have to suck it up and say good bye to your trusty lamps.

Fellow former fan and friend of fire,
Tom J.

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, OR 97701
541/693-2677
tjasper@copc.net

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Peterson, Dan
Sent: Monday, July 28, 2008 9:21 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] Alcohol lamps

Fellow Histonetters,
I am in disagreement with our lab's Safety officer with regards to
alcohol lamps. We use them in our embedding area to keep our forceps
clean.
The officer says that they're a fire hazard (even though we've used them
without incident for over 30 yrs). There are no flammable reagents
(other than the alcohol in the lamp) near the embedding area. I know we
could use the warming wells on the embedders, but try to find more that
1 pair of forceps that you like, or better yet, try to find a forceps
that the tech before hasn't left paraffin all over it. (Yes, I am a
fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY
touches)
Petty issue? Are there others out there using lamps? I am willing to
change if necessary (or so ordered), but would like to hear from those
who do the work, not be told what to do by those who know nothing of the
work. Thanks in advance!!

Daniel R Peterson HT(ASCP)
Histopathology Section Head
Meriter Laboratories
(608)-417-6557
1dpeterson@meriter.com

CONFIDENTIALITY NOTICE: This message (including any attachments) is
intended for the sole use of the individual and entity to whom it is
addressed. This message may contain information that is confidential and
is protected by law. If you are not the intended recipient, you are
hereby notified that any disclosure, copying or distribution of this
message is strictly prohibited. If you received this message in error,
please immediately notify the sender by reply email and then delete the
message. Thank you.




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 5
Date: Mon, 28 Jul 2008 14:20:34 -0400
From: "Okland, Gloria A" 
Subject: [Histonet] Blue Zones
To: 
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

Hi All, 
 
We have been seeing a very random appearance of Blue Zones on our H&E
slides.  It affects one section, but not the next in serial sections and
if we restain the slides, they look better, and it seems to just be the
GI biopsies that we have trouble with.  It seems like the tissue is not
being stained by the eosin in these areas.  The slides are
deparraffinized and hydrated, and the hematoxylin stain looks ok, it is
just the Eosin.  Any suggestions that you may have, are greatly
appreciated, this have been an ongoing problem for some time now.
 
Thanks
Gloria
 
 

Gloria Okland HT (ASCP) 
Histology Supervisor 
Quest Diagnostics 
4770 Regent Blvd 
Irving, TX 75063 
972-536-8820 
972-916-3539 fax 

 


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------------------------------

Message: 6
Date: Mon, 28 Jul 2008 18:29:51 +0000
From: sharon.osborn@comcast.net
Subject: [Histonet] alcohol lamps
To: histonet@pathology.swmed.edu
Message-ID:
	<072820081829.13697.488E101F00063A1F000035812215593414029D010D9C01D202019D0E089C@comcast.net>
	


Dan,
    About ten years ago, in setting up a new histology laboratory, management, techs, pathologists and I had this discussion.  Safety regulations require no open flames in a laboratory area due to the explosion capabilities as well as the fire hazards.  I had already told the techs we would not be using the alcohol lamps and we did not install gas lines in the new facility build out.  This was already determined by the pathology group before the building commenced.  
   There were 2-3 older techs (like me) who preferred to use the flames to clear the forceps of contamination from tissue and paraffin before embedding a new block.  Yes, it is faster, however, very dangerous.  i, too had done it for millenimum as you w/o risk.  Management thought they would get on the good side of the techs and surprise them with alcohol burners.  I discovered this before the techs came in and removed the alcohol lamps.  Thank goodness, the head pathologist backed me up.  As a post-doc he had a horrific personal experience in having an alcohol lamp ignite his lab coat when he accidentally brushed his sleeve across it.  He understood the implications of the open flame in the lab.  Also, where there  are alcohols,  xylenes or other similar flammable solvents around, the vapors could accidentally build up to create an easily combustible  situation.  So, the techs learned to use lots of Kim Wipes on the forceps before placing them in the warmers and to use Q-tips to k
eep the warmers clear of tissue pieces that could contaminate a block.  And, over time, it really does not slow you down that much in the embedding process.
    It is a change to not use the alcohol burners; however, the safety benefits far outweigh the hazards imposed by the open flame.  And, as is often the case, the embedding person may be the only one in the laboratory EARLY in the morning and no one would know there was a fire until it might be too late.

Sharon Osborn, BS, HT(ASCP) C
Lab Vision
Fremont, CA



Date: Mon, 28 Jul 2008 11:21:28 -0500
From: "Peterson, Dan" <1dpeterson@meriter.com>
Subject: [Histonet] Alcohol lamps
To: 
Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01>
Content-Type: text/plain;	charset="us-ascii"

Fellow Histonetters,
I am in disagreement with our lab's Safety officer with regards to
alcohol lamps. We use them in our embedding area to keep our forceps
clean.
The officer says that they're a fire hazard (even though we've used them
without incident for over 30 yrs). There are no flammable reagents
(other than the alcohol in the lamp) near the embedding area. I know we
could use the warming wells on the embedders, but try to find more that
1 pair of forceps that you like, or better yet, try to find a forceps
that the tech before hasn't left paraffin all over it. (Yes, I am a
fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY
touches)
Petty issue? Are there others out there using lamps? I am willing to
change if necessary (or so ordered), but would like to hear from those
who do the work, not be told what to do by those who know nothing of the
work. Thanks in advance!!

Daniel R Peterson HT(ASCP)
Histopathology Section Head
Meriter Laboratories
(608)-417-6557
1dpeterson@meriter.com



------------------------------

Message: 7
Date: Mon, 28 Jul 2008 12:48:33 -0600
From: "Gayle Callis" 
Subject: Re: [Histonet] Alcohol lamps
To: "Peterson, Dan" <1dpeterson@meriter.com>,
	
Message-ID: <001b01c8f0e2$89846710$6401a8c0@Sunney>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

We don't use open flames i.e. alcohol burners anymore, due to fire hazard 
(one could tip it over, spill alcohol and burn!).  The forcep tips, if held=20
too long in the flame, can also burn the tissue.

We use heated embedding center wells but  purchase longer forceps i.e. 
ergonomically acceptable  You can buy forceps with L,hockey stick (packing=20
forceps) shape to them, or buy straight with both wider and narrower, but 
NOT sharp pointed ends.   We keep more one forceps to choose from in our 
wells, users can chose their favorites out this selection .

We like longer, slender forceps for following reasons:
    1.  do not burn fingers during embedding
    2.  manipulate small tissues easily


Try Arista  Surgical Supply (www.aristasurgical.com ,  EMS, and Henry Schein 
(dentristy forceps, packing style) - none of these companies will break the=20
bank and Arista has a HUGE selection of all types of forceps.

As for paraffin on forceps, after embedding, lay them in cassette holding 
area on paper towel to soak up melted paraffin.  Wipe them off while hot, 
return to wells when needed.  In our lab, we have as many as 10 or more 
different people embedding their samples and THEY learn to do this very 
quickly.  No moe complaining about paraffin all over the forceps this way.=20
One can also try the metal mold holding area - where temp may be hot enought 
to melt the paraffin.   We put forceps through cleaning cycle on processor=20
and on occasion, a good paraffin removing solvent soak, rinsed off with 
alcohol or even a superhot water wash.

If you have your favorite forceps, then others must have favorites too?  Why 
not supply each person with their favorite too - then you are a hero instead 
of a "fussy old goat"?

Gayle M. Callis
 HTL/HT/MT(ASCP)

----- Original Message ----- 
From: "Peterson, Dan" <1dpeterson@meriter.com>
To: 
Sent: Monday, July 28, 2008 10:21 AM
Subject: [Histonet] Alcohol lamps


Fellow Histonetters,
I am in disagreement with our lab's Safety officer with regards to
alcohol lamps. We use them in our embedding area to keep our forceps
clean.
The officer says that they're a fire hazard (even though we've used them
without incident for over 30 yrs). There are no flammable reagents
(other than the alcohol in the lamp) near the embedding area. I know we
could use the warming wells on the embedders, but try to find more that
1 pair of forceps that you like, or better yet, try to find a forceps
that the tech before hasn't left paraffin all over it. (Yes, I am a
fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY
touches)
Petty issue? Are there others out there using lamps? I am willing to
change if necessary (or so ordered), but would like to hear from those
who do the work, not be told what to do by those who know nothing of the
work. Thanks in advance!!

Daniel R Peterson HT(ASCP)
Histopathology Section Head
Meriter Laboratories
(608)-417-6557
1dpeterson@meriter.com

CONFIDENTIALITY NOTICE: This message (including any attachments) is
intended for the sole use of the individual and entity to whom it is
addressed. This message may contain information that is confidential and
is protected by law. If you are not the intended recipient, you are
hereby notified that any disclosure, copying or distribution of this
message is strictly prohibited. If you received this message in error,
please immediately notify the sender by reply email and then delete the
message. Thank you.




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

Message: 8
Date: Mon, 28 Jul 2008 15:01:12 -0400
From: "Monfils, Paul" 
Subject: RE: [Histonet] Alcohol lamps
To: 
Message-ID:
	<4EBFF65383B74D49995298C4976D1D5E03835C40@LSRIEXCH1.lsmaster.lifespan.org>
	
Content-Type: text/plain;	charset="iso-8859-1"

Another fussy old goat here!  Alcohol lamps have served me well for 40 years, and I don't plan on changing unless they threaten to make me go back to using an autotechnicon and embedding with a pitcher of wax!

Paul M.





------------------------------

Message: 9
Date: Mon, 28 Jul 2008 14:41:22 -0500
From: Jackie M O'Connor 
Subject: Re: [Histonet] alcohol lamps
To: sharon.osborn@comcast.net
Cc: histonet-bounces@lists.utsouthwestern.edu,
	histonet@pathology.swmed.edu
Message-ID:
	
Content-Type: text/plain; charset="US-ASCII"

In 1971 in Chicago, there was a fire in a histology lab in Chicago because=20
of an alcohol lamp.   The ignited lamp was knocked off the bench, fell to 
the floor, jar broke, all the spilled alcohol was ignited over a large 
area which went on to ignite another open container of another flammable 
on the floor (cleaning xylene, I think).   A terrible fire resulted.  Two 
techs were badly burned, since there was only one door to the lab, they 
had to run through the fire to get out (lab was on the 9th floor).   I 
don't know who they were, or how they are now - but I was the technician 
who went to work there after the fire. 
Jackie O'




sharon.osborn@comcast.net 
Sent by: histonet-bounces@lists.utsouthwestern.edu
07/28/2008 01:29 PM

To
histonet@pathology.swmed.edu
cc

Subject
[Histonet] alcohol lamps







Dan,
    About ten years ago, in setting up a new histology laboratory, 
management, techs, pathologists and I had this discussion.  Safety 
regulations require no open flames in a laboratory area due to the 
explosion capabilities as well as the fire hazards.  I had already told 
the techs we would not be using the alcohol lamps and we did not install 
gas lines in the new facility build out.  This was already determined by 
the pathology group before the building commenced. 
   There were 2-3 older techs (like me) who preferred to use the flames to=20
clear the forceps of contamination from tissue and paraffin before 
embedding a new block.  Yes, it is faster, however, very dangerous.  i, 
too had done it for millenimum as you w/o risk.  Management thought they 
would get on the good side of the techs and surprise them with alcohol 
burners.  I discovered this before the techs came in and removed the 
alcohol lamps.  Thank goodness, the head pathologist backed me up.  As a 
post-doc he had a horrific personal experience in having an alcohol lamp 
ignite his lab coat when he accidentally brushed his sleeve across it.  He=20
understood the implications of the open flame in the lab.  Also, where 
there  are alcohols,  xylenes or other similar flammable solvents around, 
the vapors could accidentally build up to create an easily combustible 
situation.  So, the techs learned to use lots of Kim Wipes on the forceps 
before placing them in the warmers and to use Q-tips to k
eep the warmers clear of tissue pieces that could contaminate a block. 
And, over time, it really does not slow you down that much in the 
embedding process.
    It is a change to not use the alcohol burners; however, the safety 
benefits far outweigh the hazards imposed by the open flame.  And, as is 
often the case, the embedding person may be the only one in the laboratory=20
EARLY in the morning and no one would know there was a fire until it might=20
be too late.

Sharon Osborn, BS, HT(ASCP) C
Lab Vision
Fremont, CA



Date: Mon, 28 Jul 2008 11:21:28 -0500
From: "Peterson, Dan" <1dpeterson@meriter.com>
Subject: [Histonet] Alcohol lamps
To: 
Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01>
Content-Type: text/plain;                charset="us-ascii"

Fellow Histonetters,
I am in disagreement with our lab's Safety officer with regards to
alcohol lamps. We use them in our embedding area to keep our forceps
clean.
The officer says that they're a fire hazard (even though we've used them
without incident for over 30 yrs). There are no flammable reagents
(other than the alcohol in the lamp) near the embedding area. I know we
could use the warming wells on the embedders, but try to find more that
1 pair of forceps that you like, or better yet, try to find a forceps
that the tech before hasn't left paraffin all over it. (Yes, I am a
fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY
touches)
Petty issue? Are there others out there using lamps? I am willing to
change if necessary (or so ordered), but would like to hear from those
who do the work, not be told what to do by those who know nothing of the
work. Thanks in advance!!

Daniel R Peterson HT(ASCP)
Histopathology Section Head
Meriter Laboratories
(608)-417-6557
1dpeterson@meriter.com

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 10
Date: Mon, 28 Jul 2008 15:43:50 -0400
From: "Robert Richmond" 
Subject: [Histonet] Re: Scale for weighing parathyroid
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

Stacy McLaughlin HT (ASCP) at Cooley Dickinson Hospital in Northampton MA asks:
>>My hospital will soon be starting parathyroid surgeries. Can anyone recommend a good ultra-sensitive scale for weighing them?<<

I asked this question several days ago, here and on the pathology
list, and got no responses. I've been researching the question today,
and I've found a balance that MAY be suitable.

What you need is a balance that will go up to about 100 grams, in
increments of 10 milligrams. (Normal parathyroids run a few tens of
milligrams, while adenomas can go well over a gram.)

I called Fisher Scientific customer service at 800-766-7000.
Their model S40152 (in the online catalog) has been replaced by their
S94792B. These balances are made for Fisher by Ohaus. The Fisher
representative said that the S94792B, also called the SLF 152, is a
simplified version of Ohaus's Ohaus Valor 3000 Xtreme V31XH202, which
costs about $400. Fisher sells the simpler model for $228. The Ohaus
customer service person (at 800-526-0659, 2, 1) wasn't familiar with
the balances Ohaus makes for Fisher.

Before you go buying anything, check if your hospital pharmacy still
has a balance. That's what I've always done with parathyroid adenomas
- not very frequent specimens - but these days a lot of small hospital
pharmacies no longer compound.

Can anyone else on Histonet weigh in (sorry about that!) with some
recommendations?

Bob Richmond
Samurai Pathologist
Knoxville TN



------------------------------

Message: 11
Date: Mon, 28 Jul 2008 14:49:14 -0500
From: "Rittman, Barry R" 
Subject: RE: [Histonet] Alcohol lamps
To: "Peterson, Dan" <1dpeterson@meriter.com>,
	
Message-ID:
	
Content-Type: text/plain;	charset="iso-8859-1"

Like most things that are potentially dangerous, alcohol lamp may be a hazard. 
It is however the person using the lamp that is often the greatest hazard.
I have seen a person stick them selves accidentally in the hand with a pencil. Should we then ban all pointed objects from the lab as they are potentially dangerous?
I believe that if the individuals using the lamps are trained and adhere to appropriate safety procedures there is no problem.
I do feel on the other hand, however that some of the alcohol lamp designs are not as stable as they should be. This can however be corrected by placing several small glass beads in the alcohol reservoir to make them less likely to tip over. 
Barry

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Peterson, Dan
Sent: Mon 7/28/2008 11:21 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] Alcohol lamps



Fellow Histonetters,
I am in disagreement with our lab's Safety officer with regards to
alcohol lamps. We use them in our embedding area to keep our forceps
clean.
The officer says that they're a fire hazard (even though we've used them
without incident for over 30 yrs). There are no flammable reagents
(other than the alcohol in the lamp) near the embedding area. I know we
could use the warming wells on the embedders, but try to find more that
1 pair of forceps that you like, or better yet, try to find a forceps
that the tech before hasn't left paraffin all over it. (Yes, I am a
fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY
touches)
Petty issue? Are there others out there using lamps? I am willing to
change if necessary (or so ordered), but would like to hear from those
who do the work, not be told what to do by those who know nothing of the
work. Thanks in advance!!

Daniel R Peterson HT(ASCP)
Histopathology Section Head
Meriter Laboratories
(608)-417-6557
1dpeterson@meriter.com

CONFIDENTIALITY NOTICE: This message (including any attachments) is
intended for the sole use of the individual and entity to whom it is
addressed. This message may contain information that is confidential and
is protected by law. If you are not the intended recipient, you are
hereby notified that any disclosure, copying or distribution of this
message is strictly prohibited. If you received this message in error,
please immediately notify the sender by reply email and then delete the
message. Thank you.




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 12
Date: Mon, 28 Jul 2008 16:25:24 -0400
From: "Smith, Allen" 
Subject: RE: [Histonet] alcohol lamps
To: 'Jackie M O'Connor' 
Cc: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID:
	
	
Content-Type: text/plain; charset="us-ascii"

I have worried about this possibility. This is why I use a stamped steel alcohol lamp. The larger fire was the result of poor communication. Before lighting, I announce: I am about to strike a match.  All solvents must be covered!  (By the way, where was that lab's fire extinguisher?)

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor
Sent: Monday, July 28, 2008 3:41 PM
To: sharon.osborn@comcast.net
Cc: histonet-bounces@lists.utsouthwestern.edu; histonet@pathology.swmed.edu
Subject: Re: [Histonet] alcohol lamps

In 1971 in Chicago, there was a fire in a histology lab in Chicago because
of an alcohol lamp.   The ignited lamp was knocked off the bench, fell to
the floor, jar broke, all the spilled alcohol was ignited over a large
area which went on to ignite another open container of another flammable
on the floor (cleaning xylene, I think).   A terrible fire resulted.  Two
techs were badly burned, since there was only one door to the lab, they
had to run through the fire to get out (lab was on the 9th floor).   I
don't know who they were, or how they are now - but I was the technician
who went to work there after the fire.
Jackie O'




sharon.osborn@comcast.net
Sent by: histonet-bounces@lists.utsouthwestern.edu
07/28/2008 01:29 PM

To
histonet@pathology.swmed.edu
cc

Subject
[Histonet] alcohol lamps







Dan,
    About ten years ago, in setting up a new histology laboratory,
management, techs, pathologists and I had this discussion.  Safety
regulations require no open flames in a laboratory area due to the
explosion capabilities as well as the fire hazards.  I had already told
the techs we would not be using the alcohol lamps and we did not install
gas lines in the new facility build out.  This was already determined by
the pathology group before the building commenced.
   There were 2-3 older techs (like me) who preferred to use the flames to
clear the forceps of contamination from tissue and paraffin before
embedding a new block.  Yes, it is faster, however, very dangerous.  i,
too had done it for millenimum as you w/o risk.  Management thought they
would get on the good side of the techs and surprise them with alcohol
burners.  I discovered this before the techs came in and removed the
alcohol lamps.  Thank goodness, the head pathologist backed me up.  As a
post-doc he had a horrific personal experience in having an alcohol lamp
ignite his lab coat when he accidentally brushed his sleeve across it.  He
understood the implications of the open flame in the lab.  Also, where
there  are alcohols,  xylenes or other similar flammable solvents around,
the vapors could accidentally build up to create an easily combustible
situation.  So, the techs learned to use lots of Kim Wipes on the forceps
before placing them in the warmers and to use Q-tips to k
eep the warmers clear of tissue pieces that could contaminate a block.
And, over time, it really does not slow you down that much in the
embedding process.
    It is a change to not use the alcohol burners; however, the safety
benefits far outweigh the hazards imposed by the open flame.  And, as is
often the case, the embedding person may be the only one in the laboratory
EARLY in the morning and no one would know there was a fire until it might
be too late.

Sharon Osborn, BS, HT(ASCP) C
Lab Vision
Fremont, CA



Date: Mon, 28 Jul 2008 11:21:28 -0500
From: "Peterson, Dan" <1dpeterson@meriter.com>
Subject: [Histonet] Alcohol lamps
To: 
Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01>
Content-Type: text/plain;                charset="us-ascii"

Fellow Histonetters,
I am in disagreement with our lab's Safety officer with regards to
alcohol lamps. We use them in our embedding area to keep our forceps
clean.
The officer says that they're a fire hazard (even though we've used them
without incident for over 30 yrs). There are no flammable reagents
(other than the alcohol in the lamp) near the embedding area. I know we
could use the warming wells on the embedders, but try to find more that
1 pair of forceps that you like, or better yet, try to find a forceps
that the tech before hasn't left paraffin all over it. (Yes, I am a
fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY
touches)
Petty issue? Are there others out there using lamps? I am willing to
change if necessary (or so ordered), but would like to hear from those
who do the work, not be told what to do by those who know nothing of the
work. Thanks in advance!!

Daniel R Peterson HT(ASCP)
Histopathology Section Head
Meriter Laboratories
(608)-417-6557
1dpeterson@meriter.com

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 13
Date: Mon, 28 Jul 2008 15:30:42 -0500
From: Jackie M O'Connor 
Subject: RE: [Histonet] alcohol lamps
To: "Smith, Allen" 
Cc: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID:
	
Content-Type: text/plain; charset="US-ASCII"

Probably outside the door in the hall.   Remember, it was 1971 - - we 
could smoke and eat in the labs then, there were no safety rules.    Heck,=20
this lab only had one door.     By the time I went to work there, they had=20
made a second egress.





"Smith, Allen"  
07/28/2008 03:25 PM

To
'Jackie M O'Connor' 
cc
"'histonet@lists.utsouthwestern.edu'" 
Subject
RE: [Histonet] alcohol lamps






I have worried about this possibility. This is why I use a stamped steel 
alcohol lamp. The larger fire was the result of poor communication. Before=20
lighting, I announce: I am about to strike a match.  All solvents must be 
covered!  (By the way, where was that lab's fire extinguisher?)

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M 
O'Connor
Sent: Monday, July 28, 2008 3:41 PM
To: sharon.osborn@comcast.net
Cc: histonet-bounces@lists.utsouthwestern.edu; 
histonet@pathology.swmed.edu
Subject: Re: [Histonet] alcohol lamps

In 1971 in Chicago, there was a fire in a histology lab in Chicago because
of an alcohol lamp.   The ignited lamp was knocked off the bench, fell to
the floor, jar broke, all the spilled alcohol was ignited over a large
area which went on to ignite another open container of another flammable
on the floor (cleaning xylene, I think).   A terrible fire resulted.  Two
techs were badly burned, since there was only one door to the lab, they
had to run through the fire to get out (lab was on the 9th floor).   I
don't know who they were, or how they are now - but I was the technician
who went to work there after the fire.
Jackie O'




sharon.osborn@comcast.net
Sent by: histonet-bounces@lists.utsouthwestern.edu
07/28/2008 01:29 PM

To
histonet@pathology.swmed.edu
cc

Subject
[Histonet] alcohol lamps







Dan,
    About ten years ago, in setting up a new histology laboratory,
management, techs, pathologists and I had this discussion.  Safety
regulations require no open flames in a laboratory area due to the
explosion capabilities as well as the fire hazards.  I had already told
the techs we would not be using the alcohol lamps and we did not install
gas lines in the new facility build out.  This was already determined by
the pathology group before the building commenced.
   There were 2-3 older techs (like me) who preferred to use the flames to
clear the forceps of contamination from tissue and paraffin before
embedding a new block.  Yes, it is faster, however, very dangerous.  i,
too had done it for millenimum as you w/o risk.  Management thought they
would get on the good side of the techs and surprise them with alcohol
burners.  I discovered this before the techs came in and removed the
alcohol lamps.  Thank goodness, the head pathologist backed me up.  As a
post-doc he had a horrific personal experience in having an alcohol lamp
ignite his lab coat when he accidentally brushed his sleeve across it.  He
understood the implications of the open flame in the lab.  Also, where
there  are alcohols,  xylenes or other similar flammable solvents around,
the vapors could accidentally build up to create an easily combustible
situation.  So, the techs learned to use lots of Kim Wipes on the forceps
before placing them in the warmers and to use Q-tips to k
eep the warmers clear of tissue pieces that could contaminate a block.
And, over time, it really does not slow you down that much in the
embedding process.
    It is a change to not use the alcohol burners; however, the safety
benefits far outweigh the hazards imposed by the open flame.  And, as is
often the case, the embedding person may be the only one in the laboratory
EARLY in the morning and no one would know there was a fire until it might
be too late.

Sharon Osborn, BS, HT(ASCP) C
Lab Vision
Fremont, CA



Date: Mon, 28 Jul 2008 11:21:28 -0500
From: "Peterson, Dan" <1dpeterson@meriter.com>
Subject: [Histonet] Alcohol lamps
To: 
Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01>
Content-Type: text/plain;                charset="us-ascii"

Fellow Histonetters,
I am in disagreement with our lab's Safety officer with regards to
alcohol lamps. We use them in our embedding area to keep our forceps
clean.
The officer says that they're a fire hazard (even though we've used them
without incident for over 30 yrs). There are no flammable reagents
(other than the alcohol in the lamp) near the embedding area. I know we
could use the warming wells on the embedders, but try to find more that
1 pair of forceps that you like, or better yet, try to find a forceps
that the tech before hasn't left paraffin all over it. (Yes, I am a
fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY
touches)
Petty issue? Are there others out there using lamps? I am willing to
change if necessary (or so ordered), but would like to hear from those
who do the work, not be told what to do by those who know nothing of the
work. Thanks in advance!!

Daniel R Peterson HT(ASCP)
Histopathology Section Head
Meriter Laboratories
(608)-417-6557
1dpeterson@meriter.com

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 14
Date: Mon, 28 Jul 2008 15:34:31 -0500
From: "Drew Sally A." 
Subject: [Histonet] Human Herpes Virus 6
To: "Histonet" 
Message-ID:
	<3F328377AF4E23438E78234752652CE105D5276A@uwhis-xchng7.uwhis.hosp.wisc.edu>
	
Content-Type: text/plain;	charset="US-ASCII"

I've been asked to find out if there are any reference labs "out there"
that offer HHV-6?
Thank you for any leads offered!

Sally Ann Drew, MT (ASCP)
sdrew@uwhealth.org

IHC/ISH Lab DB1-223, Mail Code 3224
600 Highland Ave.
Madison, WI 53792
Phone (608) 265-6596
Fax (608) 262-7174



------------------------------

Message: 15
Date: Mon, 28 Jul 2008 13:43:28 -0700
From: Victor Tobias 
Subject: Re: [Histonet] alcohol lamps
To: "Jackie M O'Connor" 
Cc: histonet-bounces@lists.utsouthwestern.edu,
	sharon.osborn@comcast.net,	histonet@pathology.swmed.edu
Message-ID: <488E2F70.5050103@pathology.washington.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

What a coincidence that it happened 100 years later in Chicago, can't 
blame it on the cow this time. http://www.thechicagofire.com/

Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor@pathology.washington.edu
206-598-2792
206-598-7659 Fax
==========================3D=======================
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use 
of the intended recipients. If you are not the intended recipient, or 
if the message has been addressed to you in error, do not read, 
disclose, reproduce, distribute, disseminate or otherwise use this 
transmission. Instead, please notify the sender by reply e-mail, and 
then destroy all copies of the message and any attachments.



Jackie M O'Connor wrote:
> In 1971 in Chicago, there was a fire in a histology lab in Chicago because 
> of an alcohol lamp.   The ignited lamp was knocked off the bench, fell to=20
> the floor, jar broke, all the spilled alcohol was ignited over a large 
> area which went on to ignite another open container of another flammable=20
> on the floor (cleaning xylene, I think).   A terrible fire resulted.  Two=20
> techs were badly burned, since there was only one door to the lab, they 
> had to run through the fire to get out (lab was on the 9th floor).   I 
> don't know who they were, or how they are now - but I was the technician=20
> who went to work there after the fire. 
> Jackie O'
>
>
>
>
> sharon.osborn@comcast.net 
> Sent by: histonet-bounces@lists.utsouthwestern.edu
> 07/28/2008 01:29 PM
>
> To
> histonet@pathology.swmed.edu
> cc
>
> Subject
> [Histonet] alcohol lamps
>
>
>
>
>
>
>
> Dan,
>     About ten years ago, in setting up a new histology laboratory, 
> management, techs, pathologists and I had this discussion.  Safety 
> regulations require no open flames in a laboratory area due to the 
> explosion capabilities as well as the fire hazards.  I had already told 
> the techs we would not be using the alcohol lamps and we did not install=20
> gas lines in the new facility build out.  This was already determined by=20
> the pathology group before the building commenced. 
>    There were 2-3 older techs (like me) who preferred to use the flames to 
> clear the forceps of contamination from tissue and paraffin before 
> embedding a new block.  Yes, it is faster, however, very dangerous.  i, 
> too had done it for millenimum as you w/o risk.  Management thought they=20
> would get on the good side of the techs and surprise them with alcohol 
> burners.  I discovered this before the techs came in and removed the 
> alcohol lamps.  Thank goodness, the head pathologist backed me up.  As a=20
> post-doc he had a horrific personal experience in having an alcohol lamp=20
> ignite his lab coat when he accidentally brushed his sleeve across it.  He 
> understood the implications of the open flame in the lab.  Also, where 
> there  are alcohols,  xylenes or other similar flammable solvents around,=20
> the vapors could accidentally build up to create an easily combustible 
> situation.  So, the techs learned to use lots of Kim Wipes on the forceps=20
> before placing them in the warmers and to use Q-tips to k
> eep the warmers clear of tissue pieces that could contaminate a block. 
> And, over time, it really does not slow you down that much in the 
> embedding process.
>     It is a change to not use the alcohol burners; however, the safety 
> benefits far outweigh the hazards imposed by the open flame.  And, as is=20
> often the case, the embedding person may be the only one in the laboratory 
> EARLY in the morning and no one would know there was a fire until it might 
> be too late.
>
> Sharon Osborn, BS, HT(ASCP) C
> Lab Vision
> Fremont, CA
>
>
>
> Date: Mon, 28 Jul 2008 11:21:28 -0500
> From: "Peterson, Dan" <1dpeterson@meriter.com>
> Subject: [Histonet] Alcohol lamps
> To: 
> Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01>
> Content-Type: text/plain;                charset="us-ascii"
>
> Fellow Histonetters,
> I am in disagreement with our lab's Safety officer with regards to
> alcohol lamps. We use them in our embedding area to keep our forceps
> clean.
> The officer says that they're a fire hazard (even though we've used them
> without incident for over 30 yrs). There are no flammable reagents
> (other than the alcohol in the lamp) near the embedding area. I know we
> could use the warming wells on the embedders, but try to find more that
> 1 pair of forceps that you like, or better yet, try to find a forceps
> that the tech before hasn't left paraffin all over it. (Yes, I am a
> fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY
> touches)
> Petty issue? Are there others out there using lamps? I am willing to
> change if necessary (or so ordered), but would like to hear from those
> who do the work, not be told what to do by those who know nothing of the
> work. Thanks in advance!!
>
> Daniel R Peterson HT(ASCP)
> Histopathology Section Head
> Meriter Laboratories
> (608)-417-6557
> 1dpeterson@meriter.com
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>   



------------------------------

Message: 16
Date: Mon, 28 Jul 2008 20:43:46 +0000
From: tbritten@aol.com
Subject: Re: [Histonet] Human Herpes Virus 6
To: "Drew Sally A." ,
	histonet-bounces@lists.utsouthwestern.edu,	"Histonet"
	
Message-ID:
	<1492695284-1217277835-cardhu_decombobulator_blackberry.rim.net-176270595-@bxe197.bisx.prod.on.blackberry>
	
Content-Type: text/plain

Hi sally; we offer the slides if that helps. I don't want this to be a commercial for us. Would you like to contact me off line? Regards tom britten
------Original Message------
From: Drew Sally A.
Sender: histonet-bounces@lists.utsouthwestern.edu
To: Histonet
Sent: Jul 28, 2008 4:34 PM
Subject: [Histonet] Human Herpes Virus 6

I've been asked to find out if there are any reference labs "out there"
that offer HHV-6?
Thank you for any leads offered!

Sally Ann Drew, MT (ASCP)
sdrew@uwhealth.org

IHC/ISH Lab DB1-223, Mail Code 3224
600 Highland Ave.
Madison, WI 53792
Phone (608) 265-6596
Fax (608) 262-7174

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Sent from my Verizon Wireless BlackBerry



------------------------------

Message: 17
Date: Tue, 29 Jul 2008 10:55:35 +1200
From: "Robert Fauck [CCDHB]" 
Subject: [Histonet] Creutz Jacob Disease Fitxtion, Hanling, and
	disposal of infected tissue
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<5587F098B7C4EE489C8BE204E9565548248D12@wn0nteml05.hiq.net.nz>
Content-Type: text/plain; charset=us-ascii

Hi Everybody
 
Can somebody give me an update on CJD fixation, handling and disposal of
CJD infected ( or suspected) tissue, please.
 
Thanking you in advance for your expertise and help.
 
Regards,
 
Robert Fauck 
Wellington Hospital, New Zealand



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(1C_S1)
 
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------------------------------

Message: 18
Date: Mon, 28 Jul 2008 16:08:46 -0700 (PDT)
From: Barbara Albert 
Subject: [Histonet] Sodium barbital buffer substitute
To: histonet@lists.utsouthwestern.edu
Message-ID: <356339.21004.qm@web63707.mail.re1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi all,
I've been asked to post this question to the list.  What do you use as a substitute for Sodium barbitol buffer in your muscle histochemistries? 
 
Thanks,
Barbara Albert
UCSF Medical Center
San Francisco


      

------------------------------

Message: 19
Date: Tue, 29 Jul 2008 09:32:47 +1000
From: "Tony Henwood" 
Subject: RE: [Histonet] Alcohol lamps
To: "Peterson, Dan" <1dpeterson@meriter.com>,
	
Message-ID: 
Content-Type: text/plain; charset="us-ascii"

There have been incidents of fires at embedding benches, the wax can
ignite (i.e. wax candles).
Also alcohol fires are invisible (refer to your famous NASCARs).

There have been at least 4 that I am aware of in Australia. It is not
something that would be well publicised since it is the embarrassment
that tends to keep it quiet!

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Peterson, Dan
Sent: Tuesday, 29 July 2008 2:21 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] Alcohol lamps


Fellow Histonetters,
I am in disagreement with our lab's Safety officer with regards to
alcohol lamps. We use them in our embedding area to keep our forceps
clean. The officer says that they're a fire hazard (even though we've
used them without incident for over 30 yrs). There are no flammable
reagents (other than the alcohol in the lamp) near the embedding area. I
know we could use the warming wells on the embedders, but try to find
more that 1 pair of forceps that you like, or better yet, try to find a
forceps that the tech before hasn't left paraffin all over it. (Yes, I
am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that
NOBODY
touches)
Petty issue? Are there others out there using lamps? I am willing to
change if necessary (or so ordered), but would like to hear from those
who do the work, not be told what to do by those who know nothing of the
work. Thanks in advance!!

Daniel R Peterson HT(ASCP)
Histopathology Section Head
Meriter Laboratories
(608)-417-6557
1dpeterson@meriter.com

CONFIDENTIALITY NOTICE: This message (including any attachments) is
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is protected by law. If you are not the intended recipient, you are
hereby notified that any disclosure, copying or distribution of this
message is strictly prohibited. If you received this message in error,
please immediately notify the sender by reply email and then delete the
message. Thank you.




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

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End of Histonet Digest, Vol 56, Issue 33
****************************************

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