[Histonet] HPylori

From:"Featherstone, Annette"



Our Pathologists like the Warthin Starry Stain for Hpylori.
Annette Featherstone, Kaleida Health 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Thursday, July 31, 2008 13:04
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 56, Issue 37

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Today's Topics:

   1. Grossing of complex specimens by PAs and/or residents in
      pathology (Luke A Perkocha)
   2. PSA_NCAM immunohistochemistry (tf)
   3. Alcian Yellow (zodiac29@comcast.net)
   4. Alcohol fixation and immuno (Martin, Erin)
   5. Re: Processing of mouse hearts (Maxim_71@mail.ru)
   6. Re: -86 freezers (Jane M Flinn)
   7. Alcian Yelllow for H.pylori (zodiac29@comcast.net)
   8. Re: Alcohol fixation and immuno (Patti Loykasek)
   9. Susie Hargrove is out of the office. (SHargrove@urhcs.org)
  10. RE: Fwd: [Histonet] Processing of mouse hearts (Monfils, Paul)
  11. Please Unsubscribe (Neehar Bhatia)
  12. Unsubscribe (Juan Gutierrez)
  13. Re: Unsubscribe (Peter Carroll)
  14. (no subject) (nealtaylor01@bellsouth.net)
  15. Alcian Yellow (zodiac29@comcast.net)
  16. Re: Alcian Yelllow for H.pylori (Gayle Callis)
  17. RE: Unsubscribe (Tony Henwood)
  18. RE: Alcian Yellow (Tony Henwood)
  19. Looking for a Position (Steven Coakley)
  20. Re: Alcohol fixation and immuno (Richard Cartun)
  21. PSLIM Slide Labeling (Victor Tobias)
  22. Basic staining questions (Keith Mc Quillan)
  23. Sakura V.I.P (Marshall, Kimberly)
  24. PSA_NCAM immunohistochemistry (tf)
  25. RE: Sakura V.I.P (Ford Royer)


----------------------------------------------------------------------

Message: 1
Date: Wed, 30 Jul 2008 10:03:50 -0700
From: "Luke A Perkocha" 
Subject: [Histonet] Grossing of complex specimens by PAs and/or
	residents in pathology
To: histonet@lists.utsouthwestern.edu
Message-ID: <6.2.3.4.2.20080730092835.0204d458@exchange.ucsf.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed

Greetings,

The use of Pathology Assistants to do virtually all of the grossing of even complex pathology specimens, under the supervision of a pathologist is becoming commonplace in larger practices, and many are convinced it provides a more consistent, higher quality gross dissection and sampling, as long as the PAs are properly qualified, trained and supervised and have pathologist back-up.

Conversely, in most pathology training programs, the residents do most or all of the grossing as part of their training (sometimes with the exception of small biopsies). However, this is counter-intuitive from a patient safety standpoint: i.e. your least experienced people, who are interrupted by other concurrent responsibilities and rotate in and out on a scheduled bases are doing the part of pathology which is most critical to patient safety and can't be replicated if done wrong.

We are considering tweaking our program in some ways to improve patient safety in the gross room and reduce errors and would like to see what others do and think. The concept being tested is that more of the grossing of complex specimens should be done by PAs and experienced residents (not fresh trainees who are trying to learn all aspects of pathology and lab at the same time and are prone to
errors) and the training of residents should also include aspects of directing a gross pathology lab (which requires knowing how to gross, but also other things), rather than just grossing alone.

Could you help with the answers to these questions if you have a residency training program in pathology:

    * Do PAs gross complex specimens?
    * Are PAs involved in training residents or vice-versa?
    * What is the salary for PAs who gross complex specimens?
    * Do you have any other innovative ways to reduce gross room errors by residents in training or to teach gross pathology to same?


Luke A. Perkocha, MD, MBA
Associate Professor of Pathology and Dermatology
Associate Director of Surgical Pathology
UC San Francisco
Helen Diller Family Comprehensive Cancer Center
1600 Divisadero Avenue, Box 1785
San Francisco, CA  94143-1785
office: 415 885-7254
cell: 415 509-6442 





------------------------------

Message: 2
Date: Thu, 31 Jul 2008 01:11:21 +0800
From: "tf" 
Subject: [Histonet] PSA_NCAM immunohistochemistry
To: "histonet" 
Message-ID: <200807310111158852149@foxmail.com>
Content-Type: text/plain;	charset="gb2312"

Dear All:

Just wonder anyone have worked on PSA_NCAM IHC of frozen sections?
How about 1:1000 mouse anti-PSA NCAM from millipore to stain 30 um brain sections?

And, do you need to add triton into the PBS, to "pore" the membrane? 
I think PSA-NCAM is distributed on the cell membrane, thus PBS may work?

Protocol:
1. Rehydration. PBS
2. Mouse-anti PSA-NCAM 1:1000  overnight + Blocking simultaneously.
3. 3* PBS
4. Goat-anti mouse conjugated with 488 fluroscein
5. 3 * PBS
6. DAPI
7. PBS wash
8. mount

Any other comments on this? 
especially the time & concentration for first antibody?
thx,

2008-07-31 



tf 


------------------------------

Message: 3
Date: Wed, 30 Jul 2008 17:16:10 +0000
From: zodiac29@comcast.net
Subject: [Histonet] Alcian Yellow
To: histonet@lists.utsouthwestern.edu 
Message-ID:
	<073020081716.1090.4890A1DA000B321A000004422215575474C7CD0C0E070B0196@comcast.net>
	
Content-Type: text/plain

Hello all, 

I wanted to get some feed back from anyone using Alcian yellow as opposed to Geimsa for staining H.Pylori. Right now we are using Gemisa for detection of H.Pylori, and are considering changing to Alcian Yellow. Just wanted your opinion of what is most preferred by the pathologists.

Thanks in Advance 

Jenny

------------------------------

Message: 4
Date: Wed, 30 Jul 2008 10:31:51 -0700
From: "Martin, Erin" 
Subject: [Histonet] Alcohol fixation and immuno
To: "histonet" 
Message-ID:
	
Content-Type: text/plain; charset=iso-8859-1

Hi everyone,
 
We occassionally get blocks from outside labs that use alcohol as the fixative.  Our pathologist told me that our immunos look pretty bad when we handle these cases in the same manner as our routine FFPE cases. He is most concerned with Ki67 and K/L.   I tried them with no antigen retrieval but got no staining at all.  Any thoughts?  We only do derm.
 
Thanks,
Erin




------------------------------

Message: 5
Date: Wed, 30 Jul 2008 22:17:18 +0400
From: Maxim_71@mail.ru
Subject: Re: [Histonet] Processing of mouse hearts
To: bturdi@gmail.com
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <1253698354.20080730221718@mail.ru>
Content-Type: text/plain; charset=us-ascii

Subat:
Here is my manual protocol for mouses tissues, that
works very well for all mouse tissues (thickness no
more than 3 mm):
1. Isopropanol 70% 30 min
2. Isopropanol 80% 30 min
3. Isopropanol 95% 30 min
4. Isopropanol 99% 30 min
5. Isopropanol:Mineral oil 5:1 50oC 60 min
6. Isopropanol:Mineral oil 2:1 50oC 60 min
7. Mineral oil 1.5 h (or overnight)
8. Paraffin 60oC 30 min
9. Paraffin 60oC 30 min
10. Paraffin 60oC 20 min
11. Paraffin 60oC 20 min.
Advantage of isopropanol consists in that ethanol
have "tide" effect and do tissues hard, but
isopropanol (IPA) not.
Mineral oil = paraffin with low molecular weight
and the infiltration is better and gentle. Mineral
oil is absolutely not toxic for personnel.
Transitions between IPA, MO and paraffin are very
gentle.
Please let me know about results.
If you need any more details please contact to me.
Best wishes,
Maxim Peshkov
Russia,
Taganrog.
                         mailto:Maxim_71@mail.ru




------------------------------

Message: 6
Date: Wed, 30 Jul 2008 14:23:40 -0400
From: Jane M Flinn 
Subject: [Histonet] Re: -86 freezers
To: sridley@gmu.edu
Cc: histonet 
Message-ID: 
Content-Type: text/plain; charset="us-ascii"


WE are interested in buying a 30 cu ft -86 upright freezer. Recommnendations appreciated. Jane


"Life is short - make haste to be kind"

Dr. Jane Flinn
Director, Biopsychology Program
George Mason University, 3F5
4400 University Dr.
Fairfax, VA  22030
Phone: 703-993-4107
Fax: 703-993-1359


----- Original Message -----
From: "Martin, Erin" 
Date: Wednesday, July 30, 2008 1:31 pm
Subject: [Histonet] Alcohol fixation and immuno

> Hi everyone,
> 
> We occassionally get blocks from outside labs that use alcohol as 
> the fixative.  Our pathologist told me that our immunos look 
> pretty bad when we handle these cases in the same manner as our 
> routine FFPE cases. He is most concerned with Ki67 and K/L.   I 
> tried them with no antigen retrieval but got no staining at all.  
> Any thoughts?  We only do derm.
> 
> Thanks,
> Erin
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

------------------------------

Message: 7
Date: Wed, 30 Jul 2008 18:33:44 +0000
From: zodiac29@comcast.net
Subject: [Histonet] Alcian Yelllow for H.pylori
To: histonet@lists.utsouthwestern.edu 
Message-ID:
	<073020081833.14431.4890B40800030A6B0000385F2215578674C7CD0C0E070B0196@comcast.net>
	
Content-Type: text/plain


Rosa and Ellen, Thank you for your quick responses : )
Does anyone happen to have the procedure for Alcian yellow for H.Pylori?

Thanks again
Jenny

------------------------------

Message: 8
Date: Wed, 30 Jul 2008 11:38:14 -0700
From: Patti Loykasek 
Subject: Re: [Histonet] Alcohol fixation and immuno
To: "Martin, Erin" ,	histonet
	
Message-ID: 
Content-Type: text/plain;	charset="US-ASCII"

HI Erin. The best situation would be you could speak with this client, and
persuade them to use formalin, too. Let them know your IHC protocols are
optimized for FFPE (probably H&E's would look better, too). If this doesn't
work, obtain some alcohol fixed control  material and optimize your IHC for
this material, too. You will need to try a variety of pretreatments &
titers.  In essence you will have 2 protocols for your IHC - 1 for FFPE & 1
for alcohol fixed. What fun! Good luck with working all this out.


Patti Loykasek BS, HTL, QIHC
Clinical IHC Supervisor
PhenoPath Laboratories
Seattle, WA





> Hi everyone,
> 
> We occassionally get blocks from outside labs that use alcohol as the
> fixative.  Our pathologist told me that our immunos look pretty bad when we
> handle these cases in the same manner as our routine FFPE cases. He is most
> concerned with Ki67 and K/L.   I tried them with no antigen retrieval but got
> no staining at all.  Any thoughts?  We only do derm.
> 
> Thanks,
> Erin
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 



This e-mail message, including any attachments, is for the sole use of the 
intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S=2EA. 
at (206) 374-9000.




------------------------------

Message: 9
Date: Wed, 30 Jul 2008 13:43:22 -0500
From: SHargrove@urhcs.org
Subject: [Histonet] Susie Hargrove is out of the office.
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=US-ASCII


I will be out of the office starting  07/30/2008 and will not return until
08/04/2008.

I will respond to your message when I return. If  immediate assistance is
needed please call 3198.




------------------------------

Message: 10
Date: Wed, 30 Jul 2008 15:13:20 -0400
From: "Monfils, Paul" 
Subject: RE: Fwd: [Histonet] Processing of mouse hearts
To: "subat turdi" ,	"Derek Papalegis"
	
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	<4EBFF65383B74D49995298C4976D1D5E03835C43@LSRIEXCH1.lsmaster.lifespan.org>
	
Content-Type: text/plain;	charset="iso-8859-1"



> ----------
> From: 	histonet-bounces@lists.utsouthwestern.edu on behalf of Derek Papalegis
> Sent: 	Wednesday, July 30, 2008 12:52 PM
> To: 	subat turdi
> Cc: 	histonet@lists.utsouthwestern.edu
> Subject: 	Re: Fwd: [Histonet] Processing of mouse hearts
> 
> This is a common problem with mouse tissue. The tissue is being over 
> processed. There are a few things that you can do to remedy this. One is 
> to have a dedicated processing schedule for each different tissue that 
> you process. Although this is ideal, it is often times impossible to 
> realistically do this. If your lab is processing a variety of different=20
> mouse tissues at the same time, you will frequently encounter over 
> processed tissue. Although not ideal, a way to get good sections is to 
> rough into the blocks and before you put them on your cold tray, place 
> them in your heated water bath for a few seconds. This will rehydrate 
> the tissue enough so you can get good sections. If you do this often 
> enough, you will get a "feel" for how long you need to leave them in 
> your water bath.
> 
> It seems like you have a few unnecessary steps in how you prepare these=20
> hearts. Is there a specific reason why you use cold PBS to rinse the 
> blood out of the heart? Wouldn't formalin rinse the blood off just as 
> effectively as PBS while also starting the fixation process more 
> quickly? What is your reasoning for fixing at 4 degrees instead of at 
> room temp? Lowering the temp slows down the fixation process. The 30 
> minute running water rinse after fixation is unnecessary as well.
> 
> Feel free to contact me if you have additional questions.
> Derek
> 
> Derek Papalegis HT (ASCP)
> Histotechnician
> Division of Laboratory Animal Medicine
> Tufts University 
> 136 Harrison Avenue
> Boston, MA 02111
> phone: 617 636-2971
> fax: 617 636-8354
> 
> 
> 
> subat turdi wrote:
> > Folks,  I appreciate your responses very much.
> > This is the protocol I use.  Please give me some advice what has gone wrong.
> > Animals weighed and anesthetized  w/ ketamine-xylazine combo.Chest cavity
> > opened quickly and the heart was exposed. IVC was found and KCl was injected
> > iv to arrest the  heart was in diastole.The heart was taken out fast and
> > placed in a cold PBS to wash out the excess blood. (Perfusion w/ PFA through
> > aorta is optional) .The heart was cut into several ~2 mm thick doughnuts
> > with special interest on the ventriculum then put into 4% PFA solution for
> > 24h at 4 degrees.Then the tissue was rinsed with running water for
> > 30min.Then subjected to graded alcohols.
> >
> > 70% 30min
> >
> > 95% 20min X2
> >
> > 100% 20minX2
> >
> > Xylene 20min
> >
> > Xylene 20 min
> > Infiltrate in paraplast:
> > Station 1: 45min
> > Station 2 : 45min
> > Embedding with paraplast
> >
> > My major problem is that a lot of times I get dry blocks. The ribbons i get
> > often comes without the tissue and breaks into shards.
> >
> > Subat
> >
> >
> >  On 7/30/08, Gayle Callis  wrote:
> >   
> >> If you tell us how YOU do it, then we can help modify your protocol.
> >> ----- Original Message ----- From: "subat turdi" 
> >> To: 
> >> Sent: Tuesday, July 29, 2008 9:31 PM
> >> Subject: [Histonet] Processing of mouse hearts
> >>
> >>
> >>  Dear all,
> >>     
> >>> Does anyone have a detailed protocol for processing mouse hearts for
> >>> paraffin slides? I am new to this technique and having various troubles in
> >>> getting good histology. We don't have a automatic processer and all is
> >>> manual. Thanks in advance.
> >>>
> >>> Subat  Turdi
> >>>
> >>> School of Pharmacy
> >>> University of Wyoming
> >>> Laramie, Wyoming
> >>> _______________________________________________
> >>> Histonet mailing list
> >>> Histonet@lists.utsouthwestern.edu
> >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >>>
> >>>       > 
> >>     
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >   
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 


------------------------------

Message: 11
Date: Wed, 30 Jul 2008 14:23:48 -0500
From: Neehar Bhatia 
Subject: [Histonet] Please Unsubscribe
To: "histonet@lists.utsouthwestern.edu"
	
Message-ID: <20080730142348531.00000000760@derm-7W5SGF1>
Content-Type: text/plain; charset=us-ascii

Please unsubscribe

Thanks

 

Neehar Bhatia

Assistant Scientist

Department of Dermatology

1300 University Avenue, Rm 445

Madison WI 53706

Ph. 608.263.7144

Fax. 608.263.5223

 



------------------------------

Message: 12
Date: Wed, 30 Jul 2008 14:36:42 -0500
From: "Juan Gutierrez" 
Subject: [Histonet] Unsubscribe
To: 
Message-ID: <46B933DABE5D477A9221C2DFDD634909@precisionpath.lcl>
Content-Type: text/plain;	charset="us-ascii"

Please unsubscribe me from the list.

 

Thank you,

 

Juan C. Gutierrez, HT(ASCP)



------------------------------

Message: 13
Date: Wed, 30 Jul 2008 15:46:33 -0400
From: Peter Carroll 
Subject: Re: [Histonet] Unsubscribe
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <4890C519.6000307@umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Just for the record... do all of you "unsubscribers" know that you can 
click the link that's at the bottom of every email from this list and 
unsubscribe yourself? Just a tip!

http://lists.utsouthwestern.edu/mailman/listinfo/histonet



Juan Gutierrez wrote:
> Please unsubscribe me from the list.
>
>  
>
> Thank you,
>
>  
>
> Juan C. Gutierrez, HT(ASCP)
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   




------------------------------

Message: 14
Date: Wed, 30 Jul 2008 20:23:46 +0000
From: nealtaylor01@bellsouth.net
Subject: [Histonet] (no subject)
To: Histonet@lists.utsouthwestern.edu
Message-ID:
	<073020082023.22722.4890CDD20006E9C1000058C222216125569B0A02D2089B9A019C04040A0DBFCECF9D0104970E9B040E0A02@att.net>
	
Content-Type: text/plain

Please unsubscribe - thanks


------------------------------

Message: 15
Date: Wed, 30 Jul 2008 20:46:14 +0000
From: zodiac29@comcast.net
Subject: [Histonet] Alcian Yellow
To: histonet@lists.utsouthwestern.edu 
Message-ID:
	<073020082046.2974.4890D3160001B76500000B9E2216551406C7CD0C0E070B0196@comcast.net>
	
Content-Type: text/plain

Thank you everyone for your swift replies concerning the Alcian Yellow stain, it is much appreciated : )

Jenny

------------------------------

Message: 16
Date: Wed, 30 Jul 2008 15:04:45 -0600
From: "Gayle Callis" 
Subject: Re: [Histonet] Alcian Yelllow for H.pylori
To: ,	
Message-ID: <001c01c8f287$e4f41f90$6401a8c0@Sunney>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

You can buy the staining kits from Master Tech and probably Newcomer's or=20
Poly Scientific certainly easier than making it up, plus kits come with 
directions.  I belive staining protocols have been posted on Histonet 
several times - you can check Histonet Archives

Gayle M. Callis
HTL/HT/MT(ASCP)

----- Original Message ----- 
From: 
To: 
Sent: Wednesday, July 30, 2008 12:33 PM
Subject: [Histonet] Alcian Yelllow for H.pylori


>
> Rosa and Ellen, Thank you for your quick responses : )
> Does anyone happen to have the procedure for Alcian yellow for H.Pylori?
>
> Thanks again
> Jenny
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

Message: 17
Date: Thu, 31 Jul 2008 09:13:46 +1000
From: "Tony Henwood" 
Subject: RE: [Histonet] Unsubscribe
To: "Peter Carroll" 
Cc: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset="us-ascii"

Yep 
Nice and easy BUT

I WOULD NEVER CONSIDER IT

Histonet is too good!

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peter
Carroll
Sent: Thursday, 31 July 2008 5:47 AM
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Unsubscribe


Just for the record... do all of you "unsubscribers" know that you can 
click the link that's at the bottom of every email from this list and 
unsubscribe yourself? Just a tip!

http://lists.utsouthwestern.edu/mailman/listinfo/histonet



Juan Gutierrez wrote:
> Please unsubscribe me from the list.
>
>  
>
> Thank you,
>
>  
>
> Juan C. Gutierrez, HT(ASCP)
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   


_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
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------------------------------

Message: 18
Date: Thu, 31 Jul 2008 09:41:31 +1000
From: "Tony Henwood" 
Subject: RE: [Histonet] Alcian Yellow
To: , 
Message-ID: 
Content-Type: text/plain; charset="us-ascii"

I never saw any!

Why?

Please use "reply to all" so that everyone on the list can see your
contributions.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
zodiac29@comcast.net
Sent: Thursday, 31 July 2008 6:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Alcian Yellow


Thank you everyone for your swift replies concerning the Alcian Yellow
stain, it is much appreciated : )

Jenny
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*********************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************




------------------------------

Message: 19
Date: Wed, 30 Jul 2008 18:38:51 -0700 (PDT)
From: Steven Coakley 
Subject: [Histonet] Looking for a Position
To: histonet@lists.utsouthwestern.edu
Message-ID: <62903.6139.qm@web38207.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I'm looking for a full time HT position im the Madison, WI area.  Any information, especially research facilities that employ HT's would be appreciated.
 
Thanks,
 
 


      

------------------------------

Message: 20
Date: Thu, 31 Jul 2008 10:45:59 -0400
From: "Richard Cartun" 
Subject: Re: [Histonet] Alcohol fixation and immuno
To: "histonet" ,	"Erin Martin"
	
Message-ID: <489197E7020000770000484A@gwmail6.harthosp.org>
Content-Type: text/plain; charset=US-ASCII

Are they using pure alcohol for fixation?  I do IHC for a  dermpath lab that uses alcoholic formalin and their tissue stains very well.  The only difference is we have had to cut-back on our antigen retrieval for certain proteins.

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Martin, Erin"  07/30/08 1:31 PM >>>
Hi everyone,
 
We occassionally get blocks from outside labs that use alcohol as the fixative.  Our pathologist told me that our immunos look pretty bad when we handle these cases in the same manner as our routine FFPE cases. He is most concerned with Ki67 and K/L.   I tried them with no antigen retrieval but got no staining at all.  Any thoughts?  We only do derm.
 
Thanks,
Erin


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Message: 21
Date: Thu, 31 Jul 2008 07:55:07 -0700
From: Victor Tobias 
Subject: [Histonet] PSLIM Slide Labeling
To: Histonet 
Message-ID: <4891D24B.1060800@pathology.washington.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Does anyone have the Pslim slide labeler from AccuPlace. 
http://www.accuplace.com/PSLIM.asp It sounds really nice for our work 
environment, but I'd like some real world feedback.

Victor

-- 
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor@pathology.washington.edu
206-598-2792
206-598-7659 Fax
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Message: 22
Date: Thu, 31 Jul 2008 16:23:06 +0100
From: "Keith Mc Quillan" 
Subject: [Histonet] Basic staining questions
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<94847270807310823p72b56af7vd1481f58a1641edb@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi,

I am pretty new to staining (as in i have only ever tried it once) and would
appreciate any help you can give me with regard to processing my tissue and
staining. I will be processing mouse brains in the coming weeks with the
intention of staining for amyloid, and want to check about the correct
method for fixing this tissue. In our lab, we have always snap frozen tissue
in OCT directly after taking it, no sucrose gradients or aldehydes
etc.However, i have noticed from reading the literature that most labs seem
to fix the tissue in 4% paraformaldehyde before fixing in paraffin. Does it
make any difference as to which protocol is used? I am reluctant to change
unless i have good reason to. I am planning on perfusing the animals with
PBS before  taking half the brain for immunohistochemistry (in OCT) and the
other half for ELISA/RNA etc and so can't perfuse with PFA.

My other question relates to the staining for amyloid beta itself. I have
noticed that a number of protocols incorporate a step to treat the sections
with 70% formic acid prior to staining. Is this neccessary for the staining
of amyloid, and if so, what advantages does it offer.

Any help anyone can provide me with would be greatly appreciated

Thanks
Keith Mc Quillan
Trinity College Dublin


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Message: 23
Date: Thu, 31 Jul 2008 11:34:16 -0400
From: "Marshall, Kimberly" 
Subject: [Histonet] Sakura V.I.P
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=iso-8859-1

Hello all,
 
   I am wondering if anyone has had to change a Sakura Tissue-Tek Processor from counter top or side by side, to a up-right or stand alone.  I have a very small lab and need to make room for a new processor and it will all fit if there is a way to change it.  Any advise or info would be helpful.
 
Kimberly Marshall H.T. (ASCP)
Metroplex Hospital

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recipient or an employee or agent responsible for delivering this message to the 
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Message: 24
Date: Thu, 31 Jul 2008 23:44:26 +0800
From: "tf" 
Subject: [Histonet] PSA_NCAM immunohistochemistry
To: "histonet" 
Message-ID: <200807312344210286206@foxmail.com>
Content-Type: text/plain;	charset="gb2312"

Dear All:

Just wonder anyone have worked on PSA_NCAM IHC of frozen sections?
How about 1:1000 mouse anti-PSA NCAM from millipore to stain 30 um brain sections?

And, do you need to add triton into the PBS, to "pore" the membrane? 
I think PSA-NCAM is distributed on the cell membrane, thus PBS may work?

Protocol:
1. Rehydration. PBS
2. Mouse-anti PSA-NCAM 1:1000  overnight + Blocking simultaneously.
3. 3* PBS
4. Goat-anti mouse conjugated with 488 fluroscein
5. 3 * PBS
6. DAPI
7. PBS wash
8. mount

Any other comments on this? 
especially the time & concentration for first antibody?
thx,

2008-07-31 



tf 


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Message: 25
Date: Thu, 31 Jul 2008 11:14:37 -0500
From: "Ford Royer" 
Subject: RE: [Histonet] Sakura V.I.P
To: "'Marshall, Kimberly'" ,
	
Message-ID: <009b01c8f328$87ca90a0$7701a80a@Ford>
Content-Type: text/plain;	charset="iso-8859-1"

You did not mention the specific model number, but I believe that I can be
of help with this. I have done it before with the "K" & "E" series
processors.  

Contact me "off-List" for more information.

~ Ford

Ford M. Royer, MT(ASCP)
Histology Product Manager
Minnesota Medical, Inc.
7177 Madison Ave. W.
Golden Valley, MN 55427-3601
CELL:  612-839-1046
Phone:  763-542-8725
Fax:  763-546-4830

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall,
Kimberly
Sent: Thursday, July 31, 2008 10:34 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sakura V.I.P

Hello all,
 
   I am wondering if anyone has had to change a Sakura Tissue-Tek Processor
from counter top or side by side, to a up-right or stand alone.  I have a
very small lab and need to make room for a new processor and it will all fit
if there is a way to change it.  Any advise or info would be helpful.
 
Kimberly Marshall H.T. (ASCP)
Metroplex Hospital

==========================3D=========================3D=========================3D
==
The information contained in this message may be privileged and/or
confidential
and protected from disclosure.  If the reader of this message is not the
intended 
recipient or an employee or agent responsible for delivering this message to
the 
intended recipient, you are hereby notified that any dissemination,
distribution 
or copying of this communication is strictly prohibited.  If you have
received this
communication in error, please notify the sender immediately by replying to
this 
message and deleting the material from any computer.

==========================3D=========================3D=========================3D
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End of Histonet Digest, Vol 56, Issue 37
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