[Histonet] Alcohol fixation and immuno

From:"Martin, Erin"

Hi everyone,
We occassionally get blocks from outside labs that use alcohol as the fixative.  Our pathologist told me that our immunos look pretty bad when we handle these cases in the same manner as our routine FFPE cases. He is most concerned with Ki67 and K/L.   I tried them with no antigen retrieval but got no staining at all.  Any thoughts?  We only do derm.

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