Good advice Re: [Histonet] Filter paper

From:"Gayle Callis"

RE: [Histonet] Filter paperAlso, this caveat applies to coffee filters too. 

Gayle M. Callis 
  ----- Original Message ----- 
  From: Bonner, Janet 
  To: Wellborn, Janci R ; Weems, Joyce ; ; Gayle Callis ; ; Mylène de Champlain ; 
  Sent: Monday, July 28, 2008 7:29 AM
  Subject: RE: [Histonet] Filter paper

  Watch out that the paper you are using does not change the acidity of your solution (we had a problem with that once using the paper towels!

  Janet L. Bonner, HTL (ASCP)
  Pathology Laboratory
  Florida Hospital Winter Park

  From: on behalf of Wellborn, Janci R
  Sent: Fri 7/25/2008 10:37 AM
  To: Weems, Joyce;; Gayle Callis;; Mylène de Champlain;
  Subject: RE: [Histonet] Filter paper

  Both help to keep costs down . . .

  -----Original Message-----
  From: [] On Behalf Of Weems, Joyce
  Sent: Thursday, July 24, 2008 5:45 PM
  To:; Gayle Callis;; Mylène de Champlain;
  Subject: RE: [Histonet] Filter paper

  And paper towels will work when you have run out of filter paper...

  -----Original Message-----
  From: [] On Behalf Of
  Sent: Thursday, July 24, 2008 5:17 PM
  To: Gayle Callis;; Mylène de Champlain;
  Subject: Re: [Histonet] Filter paper

  I sometimes use coffee filters.
  ------Original Message------
  From: Gayle Callis
  To: Mylène de Champlain
  Subject: Re: [Histonet] Filter paper
  Sent: Jul 24, 2008 4:22 PM

  What is important is not the number so much as if the paper is high retention and what is filtering speed.  Whatman #4, if I remember correctly, is a medium fast paper, and should be  ideal for filtering staining solution.  Somewhere in the passage of time, I was told not to use paper that was really slow filtering speed and high retention for filtering dye solutions as the paper may retain too much of the dye needed. Now that could be an old wife's tale, but to this day I always use a medium fast paper.

    I also purchase something other than Whatman, as companies sell
  equivalents for a much cheaper price.  Whatman 54 should also work.   Big
  companies like ThermoScientific give the equivalents for their brand as compared to Whatman papers and are certainly more economical.

  Gayle M. Callis

  ----- Original Message -----
  From: "Mylène de Champlain" 
  Sent: Wednesday, July 23, 2008 4:31 PM
  Subject: [Histonet] Filter paper


    To filter blue celestin, is what I have to use a filter paper number 1 or 4? Thank!

    Mylène de Champlain

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