After some digging into the protocol, Hrapchak and Sheehan's 2nd edition of
Theory and Practice of Histotechnology, p 199 (with original #39 cited
publication from 1971 by Lie et al) has the hematoxylin, basic fuchsin,
picric acid stain for early myocardial ischemia. It is easy to do and has
worked well for us on human tissue. It can be used for animal tissue.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
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