Hal,
Since you are working with transgenes in wound healing, I'm assuming this is animal work. What I used to use for a great control was to get a mouse from (I think Jackson labs have them-but certainly one of the major mouse vendors) that is an FVB mouse with a lacZ transgene that is under control of a Tie2 promotor. Tie2 is endothelial, so can look for beta-gal expression anywhere there is endothelial cells. So essentially 1 mouse gives you all the controls, many tissues are fantastic, you could ever need and the staining on endothelium is specific and exquisite.
Ray Koelling
PhenoPath Laboratories
Seattle, WA
-------------- Original message --------------
From: "Hawkins, Hal K."
>
>
> We are using immunohistochemistry to detect expression of
> beta-galactosidase from a transgene applied to healing wounds. We would
> really like to have a good positive control. Does anyone have a block
> they could spare for this purpose that shows selective expression of
> galactosidase, or know of a commercial source?
>
>
>
> Thanks,
>
>
>
> Hal Hawkins, UTMB Galveston
>
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