Re: [Histonet] acid alcohol with immunos

From:Rene J Buesa

  Acid alcohol can be used if you use DAB as the chromogen, but it is not necessary if you just use hematoxylin during 30 seconds and "blue" it afterwards.
  You will get enough nuclear staining and the procedure will be shortened.
  René J.

Jennifer Cresor  wrote:
  Hello all,
I have been told in the past to NOT use acid alcohol to differentiate
the hematoxylin on immuno stains and only to use a progressive
hematoxylin staining method. I do not remember the reason why. Do any of
you have experiences and opinions on this subject? Is there a chance to
harm immuno stains using acid alcohol and if so why? Thank you for your

Longview, Wa.

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