Re: [Histonet] Use of molecular sieve beads

From:Constance McManus

Ruth,My husband -- an electron Microscopist -- uses them all the time for alcohols and other reagents.  I've used them before in histology and they really do a great job, but you need to allow time for them to work.  I would add mine on a Friday morning and by Monday or Tues of the next week the reagent (I was dehydrating alcohol, not xylene) was fine.  You need to use them in a certain ratio to the volume of reagent to be deydrated.  Also, there are different kinds of Molec seives used for different purposes.  I got mine from Sigma.  call them and they can help you out. Connie McManus, HTUniversity of UtahSchool of MedicineDept. of Dermatology=0A----- Original Message ----From: "Cazares, Ruth" To: histonet@pathology.swmed.eduSent: Wednesday, July 25, 2007 8:49:03 AMSubject: [Histonet] Use of molecular sieve beadsHello histonetters,I have just read an article about molecular sieve beads being used inxylene to extract the water, and therefore extending the life of thexylene indefinitely.  This article was given to me by a cytologist. Shecame across it on the cytology equivalent to the Histonet. So my=0Aquestion is, has anybody in histology used this method to keep xylenemoisture free? And if so, do it really extend the life of the xyleneindefinitely?Any and all information is greatly appreciated!Ruth Cazares, Histology SupervisorDepartment of PathologySwedish Covenant HospitalChicago, IL 60625***  Confidentiality Statement ***This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction.Thank you for your cooperation._______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu       ____________________________________________________________________________________Need a vacation? Get great dealsto amazing places on Yahoo! Travel.
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