Re: [Histonet] Use of molecular sieve beads

From:Rene J Buesa

  Xylene is usually mixed with ethanol and some amount of water during staining, and with ethanol, water and tissue components (specialy fat) during processing, which means that it is not only water what the xylene mixes with, UNLESS you are referring to xylene just in storage before being used for any purpose.
  USED xylene (i.e. the one used in tissue processing or staining) cannot be "purified" with molecular sieve beads, and the one in storage will only requiere tighten caps and short storage times. Used xylene can be "purified" only by distillation, that will eliminate both water and ethanol, and will produce a "waste" containing oils (from animal tissues and paraffin waxes).
  I would not attemp using molecular sieve beads as a routine procedure.
  René J.

"Cazares, Ruth"  wrote:
  Hello histonetters,

I have just read an article about molecular sieve beads being used in
xylene to extract the water, and therefore extending the life of the
xylene indefinitely. This article was given to me by a cytologist. She
came across it on the cytology equivalent to the Histonet. So my
question is, has anybody in histology used this method to keep xylene
moisture free? And if so, do it really extend the life of the xylene

Any and all information is greatly appreciated!

Ruth Cazares, Histology Supervisor

Department of Pathology

Swedish Covenant Hospital

Chicago, IL 60625

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