Re: [Histonet] OCT tissuetek

From:Rene J Buesa

I used to have them for years although they were not intended to save fat. It was a tumor bank used from time to time in research, always successfully.
  As long as they don't thaw (electrical failure causing the freezer to thaw) the samples can be kept for years.
  René J.

Sonny Duong  wrote:
  Hi all,

How long can samples be kept in OCT embedding media in a 
-80 freezer before they go bad? Forever? A day? I've been 
trying to stain for intramuscular triglyceride droplets 
with Oil Red O and it seems that after a couple of days the 
tissues embedded in OCT stain weaker and eventually don't 
stain at all. Any help would be appreciated!

Sonny Duong
Green Lab
University of Virginia

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