Re: [Histonet] Microwave v conventional processing

From:Rene J Buesa

  Using the adequate protocols MW processing renders equivalent results to "conventional" tissue processing, that is the general concensus.
  The thing is that unless you use an automated MW tissue processor, a histotech will have to attend to the process and change reagents manually. 
  This can lead to 2 problems: higher exposure of the HT to (usually hot) chemicals and some degree of inconsistency in the protocol because the time in each reagent could vary slightly different between runs.
  Consider that a few minutes in a conventional protocol is a much lower percentage of the time in the reagent, than the same amount of time in a much faster protocol completed with a MW tissue processor.
  MW processing should be an option when TAT is an issue and even then there are numerous manual steps independent of the time the tissue is involved in the processing step; they are independent of the processing technology and usually count for the greater part of the total TAT.
  Under separate cover I am sending you an article of mine wher I analyze this issue.
  René J. 

"Weaver, Colin"  wrote:
  Hi - we are trying to go down the microwave route in processing but
inevitably some of our veterinary pathologists are questioning whether
microwave sections are as "good" as conventional processing. Can anyone
point me in the right direction to find any comparison done between
microwave processing and conventional overnight processing with regard
to section and staining quality.

Colin Weaver
Veterinary Laboratories Agency (VLA)

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