Re: [Histonet] ISH- Kappa / Lambda

From:"Mildred Fail"

We are in the process of testing a Benchmark, one of the products weare using on the machine is the ISH for Kappa and Lambda, The results are very imppressive. More cells are staining. There is NO background staining. It is very clean. i chose bone marrow cases that had been stained IHC for Kappa and Lambda to test with the ISH for Kappa and Lambda. even though our controls are clearly cleaner with only a few more cells staining with ISH. The pt cases run in  the last 4 weeks clearly had more cells staining with ISH. Our controls are   from bxs on previous pts chosen by the pathologist. I know after reviewing these slides I am uncomfortable with the results using IHC
Rena Fail
>>> "Nita Searcy"  07/24/07 12:12 PM >>>
Am seeking information from the group regarding the use of ISH for Kappa
and Lambda instead of immunohistochemistry. Anyone doing this


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