Hi Tammy:/

    p/-phenlyenediamine is a reducing agent used to reduce osmium and 
darken the osmophilic tissue elements. I don't think it will stain 
anything by itself.  By the way, it is a serious error to dismiss 
research simply because it is "old". How do you think we got to current 
scientific knowledge, by reinventing the wheel every time technology 
changes? Of course, if you prefer to ignore the "stone age" and make the 
same mistakes over and over again .....................

Geoff


Tammy Bailey wrote:
> Has anyone had good luck staining with 1% phenylenediamine? I will be
> staining optic nerve heads of tree shrews..Most of the papers I've found are
> from the stone age..Any help would be appreciated..
>
>  
>
> Thanks,
>
> Tammy M. Bailey
>
> University of Alabama-Birmingham
>
> Vision Science Research Center
>
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> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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>
>
>   


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff@umdnj.edu
**********************************************



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