could be the fat tissues didn't completely process. I read about a technique
a few years ago
(I can't remember the author,sorry) where you melt the block done, put the
tissue between several pieces of paper towels and gently press on the tissue
to expel any remaining xylene and re-embed. I did this for a couple of colon
cases and it worked for me. It beats reprocessing the entire block.
----- Original Message -----
Sent: Friday, July 27, 2007 10:31 AM
Subject: [Histonet] Fat issues
> Hello All,
> I have some vascular graft tissues that we have paraffin embedded and some
> of the tissues have extra-vascular fat. The practice sample I sectioned
> morning seems to section well (other than the suture issues), however the
> "blows up" as soon as I place the sections into the flotation bath. Is
> there some way to minimize this? I will try cooling down the water bath
> temperature, as it may have been a bit warm.
> Any suggestions are welcome.
> Albert C. Grobe, PhD
> International Heart Institute of Montana Foundation
> Tissue Engineering Lab, Saint Patrick Hospital
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