RE: [Histonet] acid alcohol with immunos

From:"Tony Henwood"

The only reason would be if the enzyme reaction product is soluble in
ethanol (eg AEC) or acidic solutions. But isn't Mayers acidic?
The reason could possibly be that Mayers, being progressive, can be used
as a fainter nuclear stain than the more stronger Harris's (ie stain in
Mayers for a shorter time than that usually recommended).


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
[] On Behalf Of Jennifer
Sent: Friday, 3 August 2007 7:18 AM
Subject: [Histonet] acid alcohol with immunos

Hello all,
I have been told in the past to NOT use acid alcohol to differentiate
the hematoxylin on immuno stains and only to use a progressive
hematoxylin staining method. I do not remember the reason why. Do any of
you have experiences and opinions on this subject?  Is there a chance to
harm immuno stains using acid alcohol and if so why? Thank you for your
Longview, Wa.

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