RE: [Histonet] OCT tissuetek

From:"Mickie Johnson"



Dear Sonny,

We performed IHC on frozen LN for lymphoma immunophenotyping routinely at
Sacred Heart Medical Center. We found the samples stained consistently for
up to 6 months is not thawed and stored at -70 degrees C. We saved the
tissue right on the chuck. I don't have any experience with IM trygliceride
droplets. Good Luck!

Mickie

Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
Web: www.mohshistotemp.com & www.mohslabstaffing.com 
Email: mickie25@netzero.net
 
 
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sonny Duong
Sent: Tuesday, July 24, 2007 1:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] OCT tissuetek

Hi all,

How long can samples be kept in OCT embedding media in a 
-80 freezer before they go bad?  Forever? A day?  I've been 
trying to stain for intramuscular triglyceride droplets 
with Oil Red O and it seems that after a couple of days the 
tissues embedded in OCT stain weaker and eventually don't 
stain at all.  Any help would be appreciated!

Sonny Duong
Green Lab
University of Virginia


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