RE: [Histonet] Fat issues

From:"Tony Henwood"

We used your technique many times and it works a treat.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
[] On Behalf Of Mickie
Sent: Saturday, 28 July 2007 11:12 AM
To: 'Joe Nocito';;
Subject: RE: [Histonet] Fat issues

Hello Albert, Joe and all you dedicated Histonetters! Happy Friday!

I wrote an article in the Histo-Logic about 4 years ago,  (It is in the
archives) about a very useful technique for coping with this problem.
Here it is in a nut shell.

1. Melt the block down. Save the cassette
2. Blot off all wax. Don't press as this distorts the tissue
3. Put the specimen back in its original cassette and put a lid on. 4.
Put it in the with the current days run starting in formalin. 5. Process
as usual. 6. embed as usual. 

You will find that the under processed part is now processed and the
part that was ok to begin with will not be harmed by the reprocessing.
That part was protected by the paraffin that had infiltrated it

Good Luck.

Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
Web: & 

-----Original Message-----
[] On Behalf Of Joe
Sent: Friday, July 27, 2007 9:20 AM
Subject: Re: [Histonet] Fat issues

could be the fat tissues didn't completely process. I read about a

a few years ago
(I can't remember the author,sorry) where you melt the block done, put
tissue between several pieces of paper towels and gently press on the

to expel any remaining xylene and re-embed. I did this for a couple of

cases and it worked for me. It beats reprocessing the entire block.

----- Original Message ----- 
Sent: Friday, July 27, 2007 10:31 AM
Subject: [Histonet] Fat issues

> Hello All,
> I have some vascular graft tissues that we have paraffin embedded and 
> some of the tissues have extra-vascular fat.  The practice sample I  
> sectioned this morning seems to section well (other than the suture 
> issues),  however the

> fat
> "blows up" as soon as I place the sections into the flotation  bath.  
> Is there some way to minimize this?  I will try cooling down  the 
> water bath temperature, as it may have been a bit warm.
> Any suggestions are welcome.
> Thanks,
> Albert
> Albert C.  Grobe, PhD
> International Heart Institute of Montana Foundation
> Tissue  Engineering Lab, Saint Patrick  Hospital
> ************************************** Get a sneak peek of the all-new


> at
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> Histonet mailing list

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