Can someone please send me a protocol for labeling anti-human  
Neurturin (NTN) on Zamboni fixed cryostat
40um sections (free-floating). We have tried using the NTN antibody  
from RD Systems (polyclonal anti-goat)
& Chemicon's  polyclonal without success.

Both antibodies work on rat tissue (fixed in 10% NBF), but not the  
primate sections fixed in Zamboni fixative
(can't change the choice of fixative). Any information, suggestions &  
tips anyone can provide will be greatly
appreciated.

Maria Bartola Mejia
UCSF
Department of Neurosurgery
SF CA, 94103

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