[Histonet] Thin sectioning bone in PMMA

From:John Baker

Hello,  We are sectioning rat femur embedded in PMMA on the Polycut  
microtome with tungsten-carbide blade.  Does anyone have any 
suggestions/protocols for getting excellent sections at 5 microns?  Are 
there any tricks to mounting that will help eliminate the wrinkles?  We 
mount and roll on Haupt's coated Superfrost slides, then under a press 
in a 40 degree C oven overnight to dry.  Our sections are good but 
there is room for improvement.  Thanks in advance.  John

John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
109 Zina Pitcher Place, 2218 BSRB
Ann Arbor, MI 48109-2200

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