I am cutting 5 micron sections from formalin fixed, paraffin embedded
segments of rabbit abdominal aorta.
I float my ribbons in a warm water bath then pick them up with a
Superfrost Plus slide.
When I check my sections uner the microscope, the sections have a
wavy, or rippled appearance--It is not really "wrinkles, in the sense
of the paraffin folding and sticking to itself--it is as if the
arterial segment is contracting after being cut. It looks like a
circular roadway that dips down then up, then down, then up...
Has anyone ever seen this artifact with arterial sections? Any
ideas abiut why uit happens and what I could do to prevent it?
Jerry L. Ricks
U.W. Medicine at South Lake Union
815 Mercer Street
Seattle, WA 98109
See what youre getting intobefore you go there
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