Roche has PhosStop which should be added to the fixative and to all other
buffers to prevent dephosphorylation in formalin fixed tissue sections for
immunohistochemical staining. They will send you a free sample. It is
called PhosStop, a phosphatase inhibitor cocktail tablet. Catalog # is 04
906 845 001 for 10 tablets, and they have 20 tablet packages too.
At 01:06 PM 7/17/2007, you wrote:
>I know phosphoylation was kind of a hot topic at the last NSH. I will
>have to start looking at phosphoylation states in the near future. Do you
>have any suggestions?
>IHC on rat tisssues- spinal cord, brain and possibly dorsal root ganglia.
>Is perfusion or fixation preferred.
>What about frozen, how should the tissue be frozen.
>Any suggestion about buffers?
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
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