Hello All,
I have some vascular graft tissues that we have paraffin embedded and some
of the tissues have extra-vascular fat. The practice sample I sectioned this
morning seems to section well (other than the suture issues), however the fat
"blows up" as soon as I place the sections into the flotation bath. Is
there some way to minimize this? I will try cooling down the water bath
temperature, as it may have been a bit warm.
Any suggestions are welcome.
Thanks,
Albert
Albert C. Grobe, PhD
International Heart Institute of Montana Foundation
Tissue Engineering Lab, Saint Patrick Hospital
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