Re: [Histonet] Geimsa using Dif Quik

From:"Lynette Pavelich"



Hi Joyce,
Our doctors like 2 minutes in the pink and 10 minutes in the Giemsa (no
rinsing inbetween).  Then we rinse for about 10 seconds in distilled
water, air dry and coverslip.

P.S. Watch for fungus growing in the solutions.  It always shows up
before the expiration date.  We've added a thymol granule to each as a
preservative.

hope this helped,


Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503

ph:  810-257-9948
fax:  810-762-7082
>>> "Joyce Cline"  07/11/07 1:06 PM >>>
I would like to know the staining times used on Dif Quik for a Geimsa
special stain. We currently use 15 seconds for each solution. I would
like to tweak this and was wondering what everyone else was doing.


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