Jaina

Why don't you make a notch or a small slice with a razor blade on one side of the brain.  Not too big that it would interfere with the interpretation.  We do this all the time with paraffin sections on rat brain.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jaina.negandhi@sickkids.ca
Sent: Friday, July 13, 2007 8:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] c-fos staining

Hi,

I am carrying out c-fos staining on free-floating cross-sections of the brain. I am trying to map out the entire brain, and need to use a marker to distinguish between the left and right sides, as they are symmetrical.

Does anyone know of a marker (eg. dye) that I can use on a full brain sample without causing too much damage. I'd like to be able to mark it without gelatin embedding if possible.

Thanks,

Jaina Negandhi
Research Project Coordinator
Neuroscience and Mental Health
Hospital for Sick Children
Mc Master Building, RM 3006
Tel: 416-813-6551
Fax: 416-813-8724


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