ALL,
We are a research lab as well as human pathology and we "cook" our
slides for a minimum of an hour (to overnight) in a 60 degree oven prior
to IHC and special stains. For H&E they air dry until we put them on an
automated stainer in which the dryer/oven is set for 10 minutes and 65
degrees.
Unstained slides are stored at 4C and stained inside of 30 days (usually
IHC) to avoid loss of antigenicity.
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joseph
Kapler
Sent: Saturday, June 30, 2007 12:09 PM
To: Asf2k3@aol.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Histonet Digest, Vol 43, Issue 38
Depending upon the quality of the paraffin used, most blocks can now be
cut
at room temperature with very little noticeable difference to the
sectioner.
However, I agree that some tissues section better when cooled. Therefore
we
either simply refridgerate the blocks before sectioning for 15 so
minutes,
or we use the cold plate from the paraffin blocker for a minute or two.
For drying slides (I assume your referrence is for the waterbath and not
for
water in the paraffin and tissues), we gennerally leave the slides to
dry
for a couple of hours where possible. As with the earlier response
given, we
are small research/diagnostics lab and do everything manually. Once the
slides have dried (or nearly dried) we bake the slides for 15 minutes
(longer for damp slides, 30 minutes). This should be good for the
automated
system.
J.Kapler
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
Asf2k3@aol.com
Sent: Friday, June 29, 2007 22:11
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 38
1. How long should tissue paraffin blocks should be soaked in ice before
sectioning tissue with microtome?
2. How long should slides stay in oven to dry before they are put in
automated stainer?
Please let me know....
Thanks
************************************** See what's free at
http://www.aol.com.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
|