RE: [Histonet] Nuclear artefact II

From:"Marshall Terry Dr, Consultant Histopathologist"



OK.
The FS was doused in aceto-alcohol as per usual.
The pleural fluid was a cytospin (how I now hate that device) fixed in
alcohol in our normal way. (It is as you observe no doubt, air dried at
the edge from which I took the photo, but that's because the bubbles
show better there). 

Now - there's the basis of a separate thread - how can you tell whether
a smear is fixed well or not? What is "fixed well"?

Terry
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kemlo
Sent: 30 June 2007 11:33
To: Marshall Terry Dr, Consultant Histopathologist; 'Kemlo Rogerson';
lpwenk@sbcglobal.net; 'Gale, Nadia'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Nuclear artefact II

You're ignoring the question furtively:)

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall
Terry Dr,Consultant Histopathologist
Sent: 29 June 2007 14:52
To: Kemlo Rogerson; lpwenk@sbcglobal.net; Gale, Nadia;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Nuclear artefact II

You're flapping desperately:-)


Terry 

-----Original Message-----
From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk]
Sent: 29 June 2007 14:05
To: Marshall Terry Dr, Consultant Histopathologist;
lpwenk@sbcglobal.net; Gale, Nadia; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Nuclear artefact II

I thought she'd said inadequate fixation not just formalin fixation;
could it be that the frozen section and pleural fluid were inadequately
fixed? Or not fixed at all, which I suppose would prove her point,
wouldn't it?

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
 
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