I've used dako's M7249 monoclonal rat anti-mouse ki67 followed with a
biotinylated goat anti-rat with ABC detection.



Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV  89135

mtarango@nvcancer.org

Direct Line (702) 822-5112

Fax (702) 939-7663

  


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Seema
Shroff
Sent: Monday, July 02, 2007 1:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ki-67 marker for mouse CNS

Hey,

 

I'm looking to stain mouse 4% paraformaldehyde perfused, spinal cord
frozen
sections with the mitosis marker Ki-67. Does anyone know of a reliable
one
that works well in the mouse CNS and if you could please give me the
company
and catalogue number?

 

Thank you,

 

Seema Shroff

Graduate Student

Anatomy and Neurobiology

Virginia Commonwealth University.

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