[Histonet] staining query

From:"louise renton"



Hi all,  I have 2 questions to ask.

The first is...If I have counterstained my immuno too heaviliy with
haematoxylin, can I decolourise with acid alcohol & restain or will it
affect the DAB?

The second is.....Is there a  tinctorial stain I can use to differentiate
multinucleated giant cells from muscle? I cannot use immuno, as the tissue
has been badly treated (poor things) and thus IHC is muddy & yucky.

Thank you, best reagrds & have a great weekend






ent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




<< Previous Message | Next Message >>