Hello, everyone! I stained frozen and paraffin-embedded sections of human skeleton and kidney tissues using the Anti-¦Á-Dystroglycan antibody, clone VIA4-1(catalog #: 05-298) from Upstate biotechnology company by immunohistochemistry. The method is: 3um sections, EDTA 8.0 heat retrieval(pressure cooking) 10min for paraffin section£¬antibody dilution is 1:50,1:100 and 1:200(25 degree, 2 hours), and addition of EnVision antibody complex, and then DAB. But there was no any positive signals. 
   I do not know what is the matter. Please help me. Now, I need to know whether the dilution or reaction time is suitable. Please give me some good suggestions. I am eager to using the antibody smoothly. Thank you very much.
Sincerely,
 
Chunxia Zheng PhD
Research Institute of Nephrology, Jinling Hospital
305 East Zhongshan Road
Nanjing 210002, China
Phone: 86-25-80860218
Fax: 86-25-84801992

 
 
 


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