Re: [Histonet] necrosis detection

From:Rene J Buesa

  Run a little expriment by leaving unfixed tissue during at least 8 hours. Fix as usual after half an hour intervals (16 in total) and process the tissue.
  Section and stain with H&E and let your pathologist look for the signs of necrosis in each slide from each time interval. If they are not evident, increase to more time in same time length intervals.
  I think this would be a good way to find out.
  Hope this will help you!
  René J. wrote:
My pathologist has a question:

After cell death occurs, what is the earlist time at which necrosis
can be detected by H&E stains?

Angie Barnett, HTL(ASCP)
Grady Memorial Hospital
Pathology Department
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