Re: [Histonet] IHC validation of blocks and anitbodies

From:"Mildred Fail"

We submit a request  form for control material with a positive case, the pathologist approves the case , a copy of the signed request is kept in a notebook and the control logged in the control log. Everytime that block is cut the 1st and last slides are tested.
When we validate antibodies we do run a negative control. We run 13 slides 3 dilutions, 4 methods  1 negative.

Rena Fail

>>> Jessica Piche  07/24/06 12:17PM >>>
Hi All,
  I have a few questions regarding IHC. When validating controls and new antibodies do you always run a negative control slide as well? Can anyone share their protocols with me on validating controls, and new antibodies? Any help would be appreciated. Thank you.
  Jessica Piche-Grocki, HT (ASCP)
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