Re: [Histonet] Flt-1 and Flk-1
Hi Patti and Gudrun .... After extensive testing, I found that
particular rabbit polyclonal from LabVision/Neomarkers against FLT-1
to be highly unspecific. Normally I love love love the antibodies
from that company but I regret to say that I generally don't trust
any data I see using that particular anti-FLT1 Ab.
At 2:52 PM -0700 7/18/06, Patti Loykasek wrote:
>Hi Gudrun. Our research department has worked with Flt-1 about a year ago.
>For the Flt-1 (VEGF R-1) they use a rabbit polyclonal from Neomarkers with
>a heat citrate pretreatment. We do not have any experience with Flk-1.
>Hope this helps.
>Patti Loykasek BS, HTL, QIHC
>> Hi Histonetters,
>> Flt-1 and Flk-1: Is anybody successfull with ihc-staining of these two
>> antibodies (human tissue)? I use the Ventana benchmark XT (CC1 mild, 1:40,
>> 40 min).
>> Untill now I've got nuclear staining instead of cytoplasmic in placenta.
>> Any hints are appreciated.
>> Gudrun Lang
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