Re: [Histonet] Flt-1 and Flk-1

From:"Andrea T. Hooper"

Hi Patti and Gudrun .... After extensive testing, I found that 
particular rabbit polyclonal from LabVision/Neomarkers against FLT-1 
to be highly unspecific. Normally I love love love the antibodies 
from that company but I regret to say that I generally don't trust 
any data I see using that particular anti-FLT1 Ab.

At 2:52 PM -0700 7/18/06, Patti Loykasek wrote:
>Hi Gudrun. Our research department has worked with Flt-1 about a year ago.
>For the Flt-1 (VEGF R-1) they use a rabbit polyclonal   from Neomarkers with
>a heat citrate pretreatment. We do not have any experience with Flk-1.
>Hope this helps.
>Patti Loykasek BS, HTL, QIHC
>PhenoPath Laboratories
>Seattle, WA
>>  Hi Histonetters,
>>  Flt-1 and Flk-1: Is anybody successfull with ihc-staining of these two
>>  antibodies (human tissue)? I use the Ventana benchmark XT (CC1 mild, 1:40,
>>  40 min).
>>  Untill now I've got nuclear staining instead of cytoplasmic in placenta.
>>  Any hints are appreciated.
>>  Gudrun Lang
>  >

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