RE: [Histonet] sectioning thymus
"I have some thymus paraffin blocks that I am trying to section to do
> H&E on. I've never worked with this tissue before. What does it take
> to get decent sections? Most of the tissues I have cut worked fine by
> just soaking in ice with water. Sometimes I've even floated them on
> the water bath and that has helped."
I'm confused......... these are paraffin blocks aren't they? If so then why
take all that time getting the water out to soak them in the stuff again?
Thymus from memory is very "nucleary" or 'dense' and I had problems with
poorly fixed, poorly processed tissue. Solution (forgive the pun) properly
fix and then properly process; do not go near water again until the staining
part has been reached.
To cool use those plastic thingies you use to cool your tinnies.
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