RE: [Histonet] necrosis detection

From:"Marshall Terry Dr, Consultant Histopathologist"

Rene - I'm surprised at you.

Autolysis is not necrosis.

Actually to answer the original question, there is no answer.
You tell a cell is dead by its appearance, and I know not how you can tell a cell is dead until it looks dead.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire

-----Original Message-----
From: Rene J Buesa []
Sent: 18 July 2006 21:25
Subject: Re: [Histonet] necrosis detection

  Run a little expriment by leaving unfixed tissue during at least 8 hours. Fix as usual after half an hour intervals (16 in total) and process the tissue.
  Section and stain with H&E and let your pathologist look for the signs of necrosis in each slide from each time interval. If they are not evident, increase to more time in same time length intervals.
  I think this would be a good way to find out.
  Hope this will help you!
  René J. wrote:
My pathologist has a question:

After cell death occurs, what is the earlist time at which necrosis
can be detected by H&E stains?

Angie Barnett, HTL(ASCP)
Grady Memorial Hospital
Pathology Department
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