[Histonet] Request for a copy of pages on GFP visualization in FFPE tissue

From:Gayle Callis

Dear all,

If anyone has this manual, could you send a copy of Page 696 on protocol 
for fixation and paraffin embedding for GFP visualization.

  Manipulating the Mouse Embryo: A Laboratory Manual, 3rd ed.
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard
Behringer. 2003.
Cold Spring Harbor Laboratory Press.
ISBN 0-87969-574-9
page 696 for protocol on fixation and paraffin embedding for GFP visualization.

We do not have the manual in our laboratory and are doing some outside 
processing for other people.

Also, I would entertain any comments on success or lack of success on this 
subject.  I has been discussed before, but methods improve over the years 
on maintaining eGFP fluorescence after FFPE.  I would appreciate detailed 
processing schedules, in particular, use of vacuum/pressures, times in 
solvents, and paraffin temperatures.  You can send it privately if you wish.

Thanks in advance
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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