[Histonet] Postfixation of frozen brain tissue

From:"Robert J. Schloesser"

Hi All, 

We have to do IHC on fresh frozen brain samples perfused only with saline. I
am only used to cutting and IHC on PFA fixed brains. 
Therefore I have many questions on how to treat fresh frozen samples. 

Postfixation on slides: PFA or Acetone/Ethanol? 
Is there a reliable way to postfix whole blocks?
Is there a way to postfix 30-200Ám thick slides without mounting them on
slide (to do free floating staingin)? 

Any kind of input, protocols or tips&tricks and do`s and don`t are highly

Thank you very much


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