[Histonet] Postfixation of frozen brain tissue
|From:||"Robert J. Schloesser" |
We have to do IHC on fresh frozen brain samples perfused only with saline. I
am only used to cutting and IHC on PFA fixed brains.
Therefore I have many questions on how to treat fresh frozen samples.
Postfixation on slides: PFA or Acetone/Ethanol?
Is there a reliable way to postfix whole blocks?
Is there a way to postfix 30-200Ám thick slides without mounting them on
slide (to do free floating staingin)?
Any kind of input, protocols or tips&tricks and do`s and don`t are highly
Thank you very much
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