Does anyone know of a good published reference addressing various fixatives and their reactions on certain immuno's, special stains and artifacts?  I understand that there is a chance that tissue fixed in Bouin and Hollande fixatives can cause false-negative immuno results in some lymphoma markers and found another mention of a certain fixative causing a false-negative result in the Steiner and Steiner.  Also it would be great to see the pH of all the fixatives along with information such as "Do not allow Hollande's fixed specimens to come in contact with phosphate buffered formalin, a blue precipitate (artifact) occurs" - and is this what some articles refer to as being "incompatible with NBF"? 
Thanks much,
Laura

Laura Bliven
Histology Lab/IHC
Marshfield Laboratories
1000 N. Oak Ave.
Marshfield, WI 54449




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