[Histonet] Immunostaining VEGF

From:Akhil Narang

Hello everyone,

Recently subscribed to the list and I would love any
input. I am looking to stain a cell line for the
presence of VEGF and I was wondering if anyone has any
protocols or suggestions as to how to go about this. 

Is there any way to stain a cell culture without
having to fix the cells onto a slide or grow the cells
on a slide?  If so, any ideas? Also, what
antibody/secondary antibody have people had success
with? Concentrations/dilutions?

Thanks for your help.


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