[Histonet] Immunofluorescence question
Hi. Just have a few questions for those of you that do immunofluorescence.
Typically I do mostly skin punches and shaves for our dermatopath. My question
is, do any of you order the PBS that is used for the procedure or make it as
needed? I was making it up as needed, but there has been an increase in the
cases and I wondered if anyone ordered commercially. If so, please let me
know a good vendor and I will get some ordered. Nothing worse that starting to
process your specimen just to notice that someone left you an empty bottle of
PBS. I've only been doing this procedure for a few months and I am always
looking for little tips to improve my technique. So far things have been going
well. I had a problem with tissue washing off before applying the antibodies
but seem to have improved that area. I have tried applying heat, extending
drying times etc., but inevitably there is still some tissue loss. I put several
sections on each slide with the control and seem to lose usually 1 section of
the patient tissue. I would love to have all sections stay until the
coverslipping stage but maybe that's my OCD and not realistic.
Thanks for any info or words of wisdom you can share with me.
Jodi L. Putnam
St. Thomas Hospital
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