[Histonet] Giemsa stain - Helicobacter


Elisa Kelly at Kingston General Hospital, Kingston, Ontario asks about 
Diff-Quik staining of Helicobacter pylori.

Diff-Quik is a trade name. There are two staining solutions. For Helicobacter 
you need only the blue one (Diff-Quik II). There are a number of generic 
equivalents for Diff-Quik. In my personal experience they stain Helicobacter 
pylori quite as well as Diff-Quik II. Don't stain additionally with the red stain 
(Diff-Quik I) - bacteria are easier to find without it. - The only trick to 
doing the stain is to get the slide through the alcohols rapidly.

Bob Richmond
Knoxville TN and Gastonia NC
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