[Histonet] Forms of 3,3'-Diaminobenzidine (DAB)
Histonet newbie here. I recently ordered 3,3'-Diaminobenzidine (Sigma cat. # D8001), not realizing that I have always used the tetrahydrochloride form of DAB in the past, with readily dissolves in water.
I am wondering if I can simply dissolve the DAB I ordered in HCl
and perform my experiments as usual (the DAB I ordered does not dissolve in water or PBS, but does in HCl).
I used to freeze dissolved DAB in aliquots with the water-soluable form and am curious if I can do the same with the HCl-soluable form. FYI, I use it to immunohistochemically stain brain tissue.
Any advice on how to differently handle the two forms of DAB would be greatly appreciated.
Thanks so much for your input! :)
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