[Histonet] Fluorophore adsorption emission and spectral separation

From:Gayle Callis


Molecular Probes, go to resources on home page for MP, and click on their 
spectraimager.  You can play with the fluorophores you need to see (do it 
as antibodies) and see the graphs for separation - for both CLSM and 
fluorescent microscope work.

With our confocal, adjustment can be made to eliminate 
autofluorescence.   I also have a review article on autofluorescence, 
written with GFP in mind, but useful for immunofluorescent work also.

If you want the review, I will send via private email.

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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