[Histonet] Distinguishing human cells in grafts
Hi Jason & Histonetters,
We use Hoechst stain (33258) to distinguish between human and mouse
cells in grafts. It's really quick & easy, just dewax, rehydrate &
incubate in Hoechst at 4ug/ml for 1 minute, rinse with runnig water for
5 minutes, coverslip with an anti-fade mountant & look under a
fluroescene microscope. Mouse cells show discrete intranuclear
fluorescent bodies, i.e. they appear "spotty"/ punctate / grainy, but
human cells don't, i.e they appear plain.
Hope this helps,
Nic
Manchester, UK
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