It is my understanding that one of the primary applications for BAF47 is
=0Ain separating different pediatric CNS neoplasms especially rhabdoid
=0Atumors.

BAF47 identifies the "deletion" or mutation of the hSNF5/INI1 gene. (or
the corresponding reduction in protein expression at the protein level
in IHC terms). 

 

Therefore, malignant rhabdoid tumors, which can be confused with PNET's
(medulobalstomas), Ewing's, Wilm's, sarcoma, rhabdo. etc., will stain
=0A"negatively" (not stain at all) while these others would stain
positively or variably for the antibody.

 

This works very well in our lab..... we have the BAF47 antibody from BD
Transduction Labs diluted 1:200 (60 min.) with Envision+ detection (45
=0Amin.) DAB+ for 10 min.

HIER is with Tris/EDTA pH 9.0, 20 min. heat/20 min. cool.

 

The pathologists at the local Children's Hospital provided us with great
control material and are very pleased with the result!

 

See:     Am J Surg Pathol. 2004 May;28(5):644-50.

            Am J Surg Pathol. 2004 Nov;28(11):1485-91.

 

Robert
=0D 

Robert L. Lott, HTL(ASCP)

Manager, Anatomic Pathology

LabFirst / Trinity Medical Center

800 Montclair Road

Birmingham, AL   35213

205-592-5388  phone=0D
205-592-5646  fax

robert.lott@bhsala.com
=0D 




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