My experience with acetone on frozen and whole mounts is that there is 
often nonspecific fluorescence in the connective tissues. This may not 
apply to enzyme IHC but can be bad for fluorescence. Try Ethanol or 
Methanol if this happens to you.
Mel
Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm
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