AW: [Histonet] necrosis detection

From:"Gudrun Lang"

 I've allways thought, that autolysis is part of necrosis. Cell get damaged,
targeted by other cells, no oxygen or any other causes. And then the
cellmembrans brake and the hydrolases begin to "eat" up the cell. Isn't it
like this?


-----Ursprüngliche Nachricht-----
[] Im Auftrag von Marshall
Terry Dr,Consultant Histopathologist
Gesendet: Mittwoch, 19. Juli 2006 13:16
An: Rene J Buesa;;
Betreff: RE: [Histonet] necrosis detection

Rene - I'm surprised at you.

Autolysis is not necrosis.

Actually to answer the original question, there is no answer.
You tell a cell is dead by its appearance, and I know not how you can tell a
cell is dead until it looks dead.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path  Consultant
Pathologist  Rotherham General Hospital  South Yorkshire  England

-----Original Message-----
From: Rene J Buesa []
Sent: 18 July 2006 21:25
Subject: Re: [Histonet] necrosis detection

  Run a little expriment by leaving unfixed tissue during at least 8 hours.
Fix as usual after half an hour intervals (16 in total) and process the
  Section and stain with H&E and let your pathologist look for the signs of
necrosis in each slide from each time interval. If they are not evident,
increase to more time in same time length intervals.
  I think this would be a good way to find out.
  Hope this will help you!
  René J. wrote:
My pathologist has a question:

After cell death occurs, what is the earlist time at which necrosis can be
detected by H&E stains?

Angie Barnett, HTL(ASCP)
Grady Memorial Hospital
Pathology Department
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