Re: [Histonet] Alpha Smooth Muscle Actin -IHC
Use DAKO's EPOS version. It's pricey but works incredibly well with
ZERO background on mouse tissue. Although I am not familiar with the
secondary reagents you used it is likely that the background is from
the anti-mouse secondary reacting with endogenous IgG in the tissue.
If you cannot get access to EPOS you must use a mouse-on-mouse
detection system. Contact me privately if you need more information.
Hope this helps!
>Is there anyone doing IHC for alpha SMA (mouse anti-aSMA Sigma,
>clone 1A4) on mouse cryostat section ? I did on carotid artery
>tissue and I used 1/100 dilution overnight and secondary antibody is
>Histofine Max-PO (Mouse) in 1/200 dilution overnight.
>Problem is keep having background with low signal/background ratio.
>Anyone has any tips ? I keep trying for 2 months already and now it
>starts to make me frustating. Thanks before
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