RE: [Histonet] affixing large tissue blocks to glass at room temp erature
How about gelatin or agar or agarose? Put a drop on the glass, place the
blotted tissue slice on the drop, then transfer to the refrigerator or the
cold plate of the embedding unit for about a minute to gel the adhesive.
> From: firstname.lastname@example.org on behalf of
> Caroline Bass
> Sent: Tuesday, July 11, 2006 1:18 PM
> To: Histonet (E-mail)
> Subject: [Histonet] affixing large tissue blocks to glass at room
> Hey guys,
> I have several mouse liver "slices" that are fixed in NBF.
> Basically, I took a fresh liver, immersed it in NBF for about an
> hour, then sliced a lobe like a loaf of bread. I then put it in fix
> overnight to make sure the inside of the slices were well fixed. I
> now want to take "whole mount" pictures of the slices. It would help
> immensely if I could somehow fix the slices to a glass plate. The
> problem is that I want to make sure I don't destroy the slices as I
> might want to section them later. Are there any suggestions for
> temporarily affixing the slices to a glass plate at room
> temperature? Someone suggested double sided tape, but of course this
> could damage the tissue.
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